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Literature summary extracted from

  • Kusakabe, H.; Kodama, K.; Machida, H.; Midorikawa, Y.; Kuninaka, A.; Misono, H.; Soda, K.
    Occurence of a novel enzyme, L-lysine oxidase with antitumor activity in culture of Trichoderma viride (1979), Agric. Biol. Chem., 43, 337-343.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.4.3.14 medicine
-
 Trichoderma viride

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.4.3.14 extracellular
-
 Trichoderma viride
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.14 L-lysine + O2 + H2O Trichoderma viride preferred substrate 6-amino-2-oxohexanoate + NH3 + H2O2
-
 ?
1.4.3.14 L-lysine + O2 + H2O Trichoderma viride lysine metabolism 6-amino-2-oxohexanoate + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.14 Trichoderma viride
-
 mold, grown in wheat bran culture
-
1.4.3.14 Trichoderma viride
-
 Y244-2, ATCC 20536
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.14 ammonium sulfate precipitation, ion-exchange Trichoderma viride

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.14 L-arginine + O2 + H2O
-
 Trichoderma viride 5-guanidino-2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.14 L-histidine + O2 + H2O
-
 Trichoderma viride 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2
-
 ?
1.4.3.14 L-lysine + O2 + H2O preferred substrate Trichoderma viride 6-amino-2-oxohexanoate + NH3 + H2O2
-
 ?
1.4.3.14 L-lysine + O2 + H2O lysine metabolism Trichoderma viride 6-amino-2-oxohexanoate + NH3 + H2O2
-
 ?
1.4.3.14 L-ornithine + O2 + H2O
-
 Trichoderma viride 5-amino-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.14 L-phenylalanine + O2 + H2O
-
 Trichoderma viride phenylpyruvate + NH3 + H2O2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.14 37
-
 assay at Trichoderma viride

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.14 additional information
-
 heat labile Trichoderma viride
1.4.3.14 45
-
 stable for 30 min in the pH range from pH 5.0 or pH 7.0 to pH 10.0 Trichoderma viride
1.4.3.14 75
-
 30 min, complete loss of activity Trichoderma viride

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.14 7.4
-
 assay at Trichoderma viride