EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.4.3.3 | M213R | the ratio of turnover number to Km-value for D-Asp is 74fold higher than that of the wild-type enzyme, the ratio of turnover number to Km-value for D-Glu is 29.5fold higher than that of the wild-type enzyme, the ratio of turnover number to Km-value for D-Ala is 172fold lower than that of the wild-type enzyme, the ratio of turnover number to Km-value for D-Pro is 166fold lower than that of the wild-type enzyme, the ratio of turnover number to Km-value for D-Asn is 9.4fold lower than that of the wild-type enzyme, the ratio of turnover number to Km-value for D-Gln is 3.2fold higher than that of the wild-type enzyme, the ratio of turnover number to Km-value for D-Met is 56fold lower than that of the wild-type enzyme | Rhodotorula toruloides |
1.4.3.3 | M213R/Y238R | the ratio of turnover number to Km-value for D-Asp is 36.6fold higher than that of the wild-type enzyme, the ratio of turnover number to Km-value for D-Ala is 1000fold lower than that of the wild-type enzyme | Rhodotorula toruloides |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.3.1 | L-Tartrate | - |
Bos taurus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.1 | 0.2 | - |
N-Methyl-D-aspartate | - |
Bos taurus | |
1.4.3.1 | 0.9 | - |
D-proline | - |
Bos taurus | |
1.4.3.1 | 2.7 | - |
D-Aspartate | - |
Bos taurus | |
1.4.3.1 | 8.8 | - |
D-glutamate | - |
Bos taurus | |
1.4.3.3 | 0.2 | - |
D-Met | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
1.4.3.3 | 0.8 | - |
D-Ala | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
1.4.3.3 | 1.5 | - |
D-Met | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
1.4.3.3 | 2 | - |
D-Asp | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
1.4.3.3 | 3.8 | - |
D-Gln | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
1.4.3.3 | 4.6 | - |
D-Gln | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
1.4.3.3 | 11 | - |
D-Asn | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
1.4.3.3 | 14.4 | - |
D-Asn | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
1.4.3.3 | 17.8 | - |
D-Ala | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
1.4.3.3 | 18 | - |
N-Methyl-D-aspartate | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
1.4.3.3 | 18 | - |
D-Asp | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
1.4.3.3 | 21.5 | - |
D-Pro | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
1.4.3.3 | 28 | - |
D-Asp | pH 8.5, 25°C, mutant enzyme M213R/Y238R | Rhodotorula toruloides | |
1.4.3.3 | 33 | - |
D-Glu | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
1.4.3.3 | 45.8 | - |
D-Ala | pH 8.5, 25°C, mutant enzyme M213R/Y238R | Rhodotorula toruloides | |
1.4.3.3 | 77.3 | - |
D-Glu | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
1.4.3.3 | 1280 | - |
D-Pro | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.3.3 | 40000 | - |
2 * 40000, mutant enzyme M213R and wilde-type enzyme, SDS-PAGE | Rhodotorula toruloides |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.3.1 | D-aspartate + H2O + O2 | Bos taurus | - |
oxaloacetate + NH3 + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.3.1 | Bos taurus | - |
- |
- |
1.4.3.3 | Rhodotorula toruloides | P80324 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.3.3 | mutant enzyme M213R | Rhodotorula toruloides |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.4.3.1 | kidney | - |
Bos taurus | - |
EC Number | Storage Stability | Organism |
---|---|---|
1.4.3.3 | -20°C, wild-type and mutant enzyme M213R are stable for several months | Rhodotorula toruloides |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.3.1 | D-aspartate + H2O + O2 | - |
Bos taurus | oxaloacetate + NH3 + H2O2 | - |
? | |
1.4.3.1 | D-glutamate + H2O + O2 | - |
Bos taurus | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
1.4.3.1 | D-proline + H2O + O2 | - |
Bos taurus | 2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
1.4.3.1 | N-methyl-D-aspartate + H2O + O2 | - |
Bos taurus | oxaloacetate + CH3NH2 + H2O2 | - |
? | |
1.4.3.3 | D-Ala + H2O + O2 | - |
Rhodotorula toruloides | pyruvate + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-Asn + H2O + O2 | - |
Rhodotorula toruloides | 2-oxosuccinamate + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-Asp + H2O + O2 | - |
Rhodotorula toruloides | oxaloacetate + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-Glu + H2O + O2 | - |
Rhodotorula toruloides | alpha-ketoglutarate + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-glutamine + H2O + O2 | - |
Rhodotorula toruloides | 2-oxoglutaramate + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-Met + H2O + O2 | - |
Rhodotorula toruloides | 4-methylthio-2-oxobutanoate + NH3 + H2O2 | - |
? | |
1.4.3.3 | D-Pro + H2O + O2 | - |
Rhodotorula toruloides | 2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | N-methyl-D-aspartate + H2O + O2 | wild-type enzyme shows no activity, mutant enzyme M213R is active | Rhodotorula toruloides | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.3.3 | dimer | 2 * 40000, mutant enzyme M213R and wilde-type enzyme, SDS-PAGE | Rhodotorula toruloides |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.3.1 | DASPO | - |
Bos taurus |
1.4.3.3 | DAAO | - |
Rhodotorula toruloides |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.3 | 29 | - |
D-Asp | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
1.4.3.3 | 60 | - |
D-Glu | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
1.4.3.3 | 79 | - |
N-Methyl-D-aspartate | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
1.4.3.3 | 106 | - |
D-Asn | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
1.4.3.3 | 235 | - |
D-Asp | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
1.4.3.3 | 278 | - |
D-Ala | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
1.4.3.3 | 290 | - |
D-Gln | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
1.4.3.3 | 630 | - |
D-Ala | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
1.4.3.3 | 680 | - |
D-Met | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
1.4.3.3 | 975 | - |
D-Glu | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
1.4.3.3 | 1135 | - |
D-Gln | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
1.4.3.3 | 1300 | - |
D-Asn | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
1.4.3.3 | 1615 | - |
D-Pro | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
1.4.3.3 | 1665 | - |
D-Asp | pH 8.5, 25°C, mutant enzyme M213R/Y238R | Rhodotorula toruloides | |
1.4.3.3 | 4600 | - |
D-Met | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
1.4.3.3 | 4640 | - |
D-Pro | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
1.4.3.3 | 5000 | - |
D-Ala | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.3.3 | flavin | the mutant enzyme M213R has a more polar microenvironment surrounding the flavin coenzyme | Rhodotorula toruloides |