Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Coulton, J.W.; Kapoor, M.
    Purification and some properties of the glutamate dehydrogenase of Salmonella typhimurium (1973), Can. J. Microbiol., 19, 427-438.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.4 p-hydroxymercuribenzoic acid at 10 mM, 90% inhibition after 40 min Salmonella enterica subsp. enterica serovar Typhimurium
1.4.1.4 sodium dodecylsulfate at 0.7% w/v after 1 h 5% activity Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.4 280000
-
 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium
1.4.1.4 291000
-
 sucrose-density-gradient centrifugation Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.4 L-glutamate + NADP+ + H2O Salmonella enterica subsp. enterica serovar Typhimurium
-
 2-oxoglutarate + NADPH + NH3
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.4 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.4
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.4 L-glutamate + NADP+ + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium 2-oxoglutarate + NADPH + NH3
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.4 50
-
 5 min stable Salmonella enterica subsp. enterica serovar Typhimurium
1.4.1.4 65
-
 complete inactivation Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.4 8.6
-
 reductive amination Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.4 NADP+
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.4.1.4 NADPH
-
 Salmonella enterica subsp. enterica serovar Typhimurium