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Literature summary extracted from

  • Shimizu, H.; Kuratsu, T.; Hirata, F.
    Purification and some properties of glutamate dehydrogenase from Proteus inconstans (1979), J. Ferment. Technol., 57, 428-433.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.4 ADP at 0.3 mM 40% activation Proteus inconstans
1.4.1.4 ATP at 0.3 mM, 60% activation of reductive amination Proteus inconstans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.1.4 dialysis against ammonium sulfate solution Proteus inconstans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.4 ADP at 0.3 mM, 22% inhibition of oxidative deamination Proteus inconstans
1.4.1.4 AMP at 0.3 mM 33% inhibition of oxidative deamination Proteus inconstans
1.4.1.4 Hg2+ at 1 mM, 100% activity loss Proteus inconstans
1.4.1.4 iodoacetamide at 4 mM, complete inactivation Proteus inconstans
1.4.1.4 N-ethylmaleimide at 0.8 mM, 44% inhibition Proteus inconstans
1.4.1.4 p-chloromercuriphenyl sulfonate inactivetes, can be reversed by addition of cysteine Proteus inconstans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.4 0.003
-
 NADPH reductive amination Proteus inconstans
1.4.1.4 0.023
-
 NADP+ oxidative deamination Proteus inconstans
1.4.1.4 0.37
-
 NH4+ reductive amination Proteus inconstans
1.4.1.4 2.3
-
 L-glutamate oxidative deamination Proteus inconstans
1.4.1.4 3.7
-
 L-glutamate reductive amination Proteus inconstans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.4 40000
-
 x * 40000, SDS-PAGE Proteus inconstans
1.4.1.4 400000
-
 gel filtration Proteus inconstans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.4 L-glutamate + NADP+ + H2O Proteus inconstans
-
 2-oxoglutarate + NADPH + NH3
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.4 Proteus inconstans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.4
-
 Proteus inconstans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.4 231
-
 oxidative deamination at 25°C Proteus inconstans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.4 2-oxoglutarate + NH4+ + NADPH
-
 Proteus inconstans L-glutamate + NADP+
-
 ?
1.4.1.4 L-glutamate + NADP+ + H2O
-
 Proteus inconstans 2-oxoglutarate + NADPH + NH3
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.4 ? x * 40000, SDS-PAGE Proteus inconstans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.4 40
-
-
 Proteus inconstans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.4 50
-
 irreversible heat inactivation Proteus inconstans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.4 8.5
-
 reductive amination Proteus inconstans
1.4.1.4 10
-
 oxidative deamination Proteus inconstans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.1.4 6 9 unstable below pH 5.0 or above pH 10.0 Proteus inconstans

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.4 NADP+
-
 Proteus inconstans
1.4.1.4 NADPH
-
 Proteus inconstans