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Literature summary extracted from

  • Baars, J.J.P.; Op den Camp, H.J.M.; van Hoek, A.H.A.M.; van der Drift, C.; Van Griensven, L.J.L.D.; Visser, J.; Vogels, G.D.
    Purification and characterization of NADP-dependent glutamate dehydrogenase from the commercial mushroom Agaricus bisporus (1995), Curr. Microbiol., 30, 211-217.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.4 ADP at 4 mM slight inhibitory Agaricus bisporus
1.4.1.4 ATP at 4 mM slight inhibitory Agaricus bisporus
1.4.1.4 oxaloacetate at 20 mM slight inhibitory Agaricus bisporus
1.4.1.4 pyruvate at 10 mM slight inhibitory Agaricus bisporus
1.4.1.4 Urea at 0°C 1 h at 2 M 65% inhibition Agaricus bisporus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.4 0.074
-
 NADPH reductive amination at 33°C Agaricus bisporus
1.4.1.4 0.117
-
 NADP+ oxidative deamination at 33°C Agaricus bisporus
1.4.1.4 2.1
-
 NH4+ reductive amination at 33°C Agaricus bisporus
1.4.1.4 3.2
-
 2-oxoglutarate reductive amination at 33°C Agaricus bisporus
1.4.1.4 27
-
 L-glutamate oxidative deamination at 33°C Agaricus bisporus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.4 48000
-
 alpha6, 6 * 48000, SDS-PAGE Agaricus bisporus
1.4.1.4 330000
-
 gel filtration Agaricus bisporus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.4 L-glutamate + NADP+ + H2O Agaricus bisporus
-
 2-oxoglutarate + NADPH + NH3
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.4 Agaricus bisporus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.4 8.2
-
 reductive amination at 33°C Agaricus bisporus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.4 2-oxoglutarate + NH4+ + NADPH
-
 Agaricus bisporus L-glutamate + NADP+
-
 ?
1.4.1.4 L-glutamate + NADP+ + H2O
-
 Agaricus bisporus 2-oxoglutarate + NADPH + NH3
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.4 homohexamer alpha6, 6 * 48000, SDS-PAGE Agaricus bisporus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.4 34
-
 reductive amination Agaricus bisporus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.4 25
-
 12% activity loss after 1 h Agaricus bisporus
1.4.1.4 37
-
 72% activity loss after 1 h Agaricus bisporus
1.4.1.4 55
-
 complete activity loss after 1 h Agaricus bisporus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.4 7.6
-
 reductive amination Agaricus bisporus
1.4.1.4 9
-
 oxidative deamination Agaricus bisporus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.4 NADP+
-
 Agaricus bisporus
1.4.1.4 NADPH
-
 Agaricus bisporus