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Literature summary extracted from

  • Moyano, E.; Cardenas, J.; Munoz-Blanco, J.
    Purification and properties of three NAD(P)+ isozymes of L-glutamate dehydrogenase of Chlamydomonas reinhardtii (1992), Biochim. Biophys. Acta, 1119, 63-68.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.3 Aminooxyacetate 5 mM, weak inhibition of isozymes 1-3 Chlamydomonas reinhardtii
1.4.1.3 EDTA 5 mM, 80-90% inhibition of isozymes 1-3 Chlamydomonas reinhardtii
1.4.1.3 KCN 50 mM, strong inhibition of isozymes 1-3 Chlamydomonas reinhardtii
1.4.1.3 Methylacetimidate 100 mM, moderate inhibition of isozymes 1-3 Chlamydomonas reinhardtii
1.4.1.3 Ni2+ 1 mM, moderate inhibition of isozymes 1-3 Chlamydomonas reinhardtii
1.4.1.3 o-phenanthroline 5 mM, strong inhibition of isozymes 1-3 Chlamydomonas reinhardtii
1.4.1.3 p-hydroxymercuribenzoate 5 mM, moderate inhibition of isozymes 1-3 Chlamydomonas reinhardtii
1.4.1.3 pyridoxal 5'-phosphate 5 mM, 100% inhibition Chlamydomonas reinhardtii
1.4.1.3 Zn2+ 1 mM, strong inhibition of isozymes 1-3 Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.3 0.07
-
NADH isozyme 1, amination activity, cofactor NADH Chlamydomonas reinhardtii
1.4.1.3 0.09
-
NADH isozyme 3, amination activity, cofactor NADH Chlamydomonas reinhardtii
1.4.1.3 0.14
-
NADH isozyme 2, amination activity, cofactor NADH Chlamydomonas reinhardtii
1.4.1.3 0.2
-
NAD+ isozyme 1, deamination activity Chlamydomonas reinhardtii
1.4.1.3 0.26
-
NAD+ isozyme 2, deamination activity Chlamydomonas reinhardtii
1.4.1.3 0.27
-
NADPH isozyme 1, amination activity, cofactor NADPH Chlamydomonas reinhardtii
1.4.1.3 0.32
-
NAD+ isozyme 3, deamination activity Chlamydomonas reinhardtii
1.4.1.3 0.36
-
2-oxoglutarate isozyme 1, amination activity, cofactor NADH Chlamydomonas reinhardtii
1.4.1.3 0.46
-
NADPH isozyme 3, amination activity, cofactor NADPH Chlamydomonas reinhardtii
1.4.1.3 0.59
-
2-oxoglutarate isozyme 3, amination activity, cofactor NADH Chlamydomonas reinhardtii
1.4.1.3 0.64
-
L-glutamate isozyme 1, deamination activity Chlamydomonas reinhardtii
1.4.1.3 0.78
-
NADPH isozyme 2, amination activity, cofactor NADPH Chlamydomonas reinhardtii
1.4.1.3 1.23
-
2-oxoglutarate isozyme 2, amination activity, cofactor NADH Chlamydomonas reinhardtii
1.4.1.3 1.54
-
2-oxoglutarate isozyme 1, amination activity, cofactor NADPH Chlamydomonas reinhardtii
1.4.1.3 1.73
-
2-oxoglutarate isozyme 3, amination activity, cofactor NADH Chlamydomonas reinhardtii
1.4.1.3 1.85
-
2-oxoglutarate isozyme 2, amination activity, cofactor NADPH Chlamydomonas reinhardtii
1.4.1.3 3
-
L-glutamate isozyme 3, deamination activity Chlamydomonas reinhardtii
1.4.1.3 3.52
-
L-glutamate isozyme 2, deamination activity Chlamydomonas reinhardtii
1.4.1.3 13
-
NH4+ isozyme 2, amination activity, cofactor NADH Chlamydomonas reinhardtii
1.4.1.3 30
-
NH4+ isozyme 1, amination activity, cofactor NADH Chlamydomonas reinhardtii
1.4.1.3 36
-
NH4+ isozyme 1, amination activity, cofactor NADPH Chlamydomonas reinhardtii
1.4.1.3 41
-
NH4+ isozyme 3, amination activity, cofactor NADPH Chlamydomonas reinhardtii
1.4.1.3 44
-
NH4+ isozyme 3, amination activity, cofactor NADH Chlamydomonas reinhardtii
1.4.1.3 53
-
NH4+ isozyme 2, amination activity, cofactor NADPH Chlamydomonas reinhardtii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.1.3 Ca2+ 1 mM, 1.5fold activation of isozymes 1 and 3, 2.5fold activation of isozyme 2 Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.3 44000
-
4 * 44000 + 2 * 46000, isoenzyme GDH2, SDS-PAGE Chlamydomonas reinhardtii
1.4.1.3 46000
-
4 * 44000 + 2 * 46000, isoenzyme GDH2, SDS-PAGE Chlamydomonas reinhardtii
1.4.1.3 266000 269000 gel filtration Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.3 Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.3 isoenzymes, glutamate dehydrogenases 1, 2 and 3 Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.3 58
-
isozyme 3 Chlamydomonas reinhardtii
1.4.1.3 67
-
isozyme 2 Chlamydomonas reinhardtii
1.4.1.3 230
-
isozyme 1 Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.3 2-oxoglutarate + NH3 + NADH + H+
-
Chlamydomonas reinhardtii L-glutamate + H2O + NAD+
-
r
1.4.1.3 L-glutamate + H2O + NAD(P)+
-
Chlamydomonas reinhardtii 2-oxoglutarate + NH3 + NAD(P)H
-
r

Subunits

EC Number Subunits Comment Organism
1.4.1.3 hexamer 4 * 44000 + 2 * 46000, isoenzyme GDH2, SDS-PAGE Chlamydomonas reinhardtii
1.4.1.3 hexamer isoenzyme GDH1, SDS-PAGE Chlamydomonas reinhardtii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.3 60 62
-
Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.3 7.7
-
NADPH + 2-oxoglutarate + NH3 Chlamydomonas reinhardtii
1.4.1.3 8.5
-
NADH + 2-oxoglutarate + NH3 Chlamydomonas reinhardtii
1.4.1.3 9
-
NAD+ + glutamate Chlamydomonas reinhardtii