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Literature summary extracted from

  • Mc Kenzie, E.A.; Lees, E.M.
    Glutamate dehydrogenase activity in developing soybean seed: isolation and characterization of three forms of the enzyme (1981), Arch. Biochem. Biophys., 212, 290-297.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.3 2-mercaptoethanol 2fold activation Glycine max
1.4.1.3 glutathione 2fold activation Glycine max

General Stability

EC Number General Stability Organism
1.4.1.3 2-mercaptoethanol stabilizes Glycine max
1.4.1.3 glutathione stabilizes Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.4.1.3 chloroplast
-
 Glycine max 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.3 300000 350000 isoenzymes GDH1, GDH2, disc gel electrophoresis Glycine max

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.3 Glycine max
-
 glutamate dehydrogenase 1 and 2
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.1.3 leaf
-
 Glycine max
-
1.4.1.3 seed glutamate dehydrogenase 1 is found only in the developing seed Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.3 1.2
-
 glutamate dehydrogenase 2 Glycine max
1.4.1.3 1.8
-
 glutamate dehydrogenase 1 Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.3 L-glutamate + H2O + NAD(P)+
-
 Glycine max 2-oxoglutarate + NH3 + NAD(P)H
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.3 7.6
-
 NADPH Glycine max
1.4.1.3 8
-
-
 Glycine max
1.4.1.3 8.3
-
 2-oxoglutarate + NH3 + NAD(P)H Glycine max
1.4.1.3 8.7
-
 NADP+ + glutamate Glycine max
1.4.1.3 8.9
-
 NAD+ + glutamate Glycine max

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.1.3 7.5 8.8 NAD(P)H + 2-oxoglutarate + NH3, more than 50% of activity maximum at pH 7.5 and 8.8 Glycine max
1.4.1.3 8 9.2 deamination, more than 50% of activity maximum at pH 8.0 and 9.2 Glycine max

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.3 NAD+
-
 Glycine max
1.4.1.3 NADH reaction rate NADH/NADPH: 4.5 (GDH2) Glycine max
1.4.1.3 NADH reaction rate NADH/NADPH: 1.3 (GDH1) Glycine max
1.4.1.3 NADP+
-
 Glycine max
1.4.1.3 NADPH glutamate dehydrogenase 2, reaction rate NADH/NADPH: 4.4 Glycine max
1.4.1.3 NADPH glutamate dehydrogenase 1, reaction rate NADH/NADPH: 1.3 Glycine max