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Literature summary extracted from

  • Lin, M.C.M.; Wagner, C.
    Purification and characterization of N-methylalanine dehydrogenase (1975), J. Biol. Chem., 250, 3746-3751.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.17 2-mercaptoethanol required for full activity Pseudomonas sp.
1.4.1.17 dithiothreitol can replace 2-mercaptoethanol Pseudomonas sp.

General Stability

EC Number General Stability Organism
1.4.1.17 unstable in the absence of 2-mercaptoethanol or dithiothreitol Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.17 NADPH substrate inhibition above 1 mM Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.17 0.035
-
 NADPH
-
 Pseudomonas sp.
1.4.1.17 15
-
 pyruvate
-
 Pseudomonas sp.
1.4.1.17 75
-
 methylamine
-
 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.17 36500
-
 SDS-PAGE Pseudomonas sp.
1.4.1.17 77000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.17 pyruvate + methylamine + NADPH Pseudomonas sp. reverse reaction only to limited extend at very low rate N-methyl-L-alanine + H2O + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.17 Pseudomonas sp.
-
 MS ATCC 25262
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.17
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.17 0.37
-
 purified enzyme Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.17 methylamine + alpha-ketobutyrate + NADPH 14% of activity with pyruvate Pseudomonas sp. glutamic acid + H2O + NADP+
-
 ?
1.4.1.17 methylamine + oxaloacetate + NADPH 64% of activity with pyruvate Pseudomonas sp. aspartic acid + H2O + NADP+
-
 ?
1.4.1.17 pyruvate + methylamine + NADPH reverse reaction only to limited extend at very low rate Pseudomonas sp. N-methyl-L-alanine + H2O + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.17 dimer 2 * 36500, SDS-PAGE, gel filtration Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.17 8.2 8.6
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.17 NADPH
-
 Pseudomonas sp.