EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.13 | 3-Bromo-2-oxoglutarate | - |
Escherichia coli | |
1.4.1.13 | 4-Iodoacetamidosalicylate | - |
Escherichia coli | |
1.4.1.13 | Bromopyruvate | - |
Escherichia coli | |
1.4.1.13 | iodoacetamide | selective inactivation of glutamine-dependent activity | Escherichia coli | |
1.4.1.13 | L-2-Amino-4-oxo-5-chloropentanoic acid | selective inhibitor of glutamine-dependent activity | Escherichia coli | |
1.4.1.13 | N-ethylmaleimide | - |
Escherichia coli | |
1.4.1.13 | NADPH | inactivation of glutamine-dependent activity, 50% inactivation at 0.36 mM in 10 min | Escherichia coli | |
1.4.1.13 | p-chloromercuribenzoate | - |
Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.13 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.1.13 | using streptomycin sulfate treatment, ammonium sulfate fractionation, heat treatment, agarose gel filtration and DEAE-cellulose column chromatography | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.4.1.13 | 14.3 | - |
native enzyme | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.13 | L-glutamine + 2-oxoglutarate + NADPH + H+ | in the reverse reaction ammonia can act instead of glutamine, but more slowly | Escherichia coli | L-glutamate + NADP+ | - |
? | |
1.4.1.13 | NH3 + 2-oxoglutarate + NADPH + H+ | the specific activity of native enzyme using NH3 varies between 5% and 7% of the glutamine-dependent activity | Escherichia coli | L-glutamate + NADP+ | - |
? |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.13 | NADPH | - |
Escherichia coli |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.13 | 0.025 | - |
L-2-Amino-4-oxo-5-chloropentanoic acid | - |
Escherichia coli |