EC Number | General Stability | Organism |
---|---|---|
1.4.1.13 | 2-mercaptoethanol, stabilizes | Thiobacillus thioparus |
1.4.1.13 | DTT, 1 mM, stabilizes at 4°C | Thiobacillus thioparus |
1.4.1.13 | EDTA, stabilizes | Thiobacillus thioparus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.13 | 2,2'-bipyridyl | 100% inhibition at 3 mM | Thiobacillus thioparus | |
1.4.1.13 | Atebrin | 100% inhibition at 1 mM | Thiobacillus thioparus | |
1.4.1.13 | ATP | 33% inhibition at 20 mM | Thiobacillus thioparus | |
1.4.1.13 | D-glutamate | - |
Thiobacillus thioparus | |
1.4.1.13 | iodoacetate | 27% inhibition at 25 mM | Thiobacillus thioparus | |
1.4.1.13 | isocitrate | 32% inhibition at 20 mM | Thiobacillus thioparus | |
1.4.1.13 | KCN | 89% inhibition at 20 mM | Thiobacillus thioparus | |
1.4.1.13 | L-glutamate | more than 40% inhibition at 50 mM | Thiobacillus thioparus | |
1.4.1.13 | L-histidine | more than 40% inhibition at 50 mM | Thiobacillus thioparus | |
1.4.1.13 | L-methionine | more than 40% inhibition at 50 mM | Thiobacillus thioparus | |
1.4.1.13 | L-serine | more than 40% inhibition at 10 mM and 50 mM | Thiobacillus thioparus | |
1.4.1.13 | additional information | a combination of L-serine, L-methionine, L-alanine, L-glycine, L-histidine and L-asparagine inhibits 73% of the acitivity at 7.5 mM each | Thiobacillus thioparus | |
1.4.1.13 | N-ethylmaleimide | 83% inhibition at 10 mM | Thiobacillus thioparus | |
1.4.1.13 | NADP+ | 59% inhibition at 1 mM | Thiobacillus thioparus | |
1.4.1.13 | NaN3 | 100% inhibition at 50 mM, 11% inhibition at 20 mM | Thiobacillus thioparus | |
1.4.1.13 | o-phenanthroline | 37% inhibition at 10 mM | Thiobacillus thioparus | |
1.4.1.13 | oxaloacetate | more than 50% inhibition at 20 mM | Thiobacillus thioparus | |
1.4.1.13 | p-hydroxymercuribenzoate | complete inhibition at 1 mM, 5 min | Thiobacillus thioparus | |
1.4.1.13 | sodium arsenite | 100% inhibition at 50 mM , 10% inhibition at 20 mM | Thiobacillus thioparus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.13 | 0.003 | - |
NADPH | - |
Thiobacillus thioparus | |
1.4.1.13 | 0.05 | - |
alpha-ketoglutarate | - |
Thiobacillus thioparus | |
1.4.1.13 | 1.1 | - |
L-glutamine | - |
Thiobacillus thioparus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.1.13 | 72000 | - |
x * 72000 + x * 94000 | Thiobacillus thioparus |
1.4.1.13 | 94000 | - |
x * 72000 + x * 94000 | Thiobacillus thioparus |
1.4.1.13 | 280000 | - |
gel filtration | Thiobacillus thioparus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.13 | Thiobacillus thioparus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.1.13 | using ammonium sulfate fractionation, column chromatography on DEAE-cellulose and Sephadex G-200 | Thiobacillus thioparus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.4.1.13 | additional information | - |
- |
Thiobacillus thioparus |
1.4.1.13 | 9.3 | - |
- |
Thiobacillus thioparus |
EC Number | Storage Stability | Organism |
---|---|---|
1.4.1.13 | storing at -20°C, total loss of activity after 10 days | Thiobacillus thioparus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.13 | L-glutamine + 2-oxoglutarate + NADPH + H+ | in the reverse reaction ammonia can act instead of glutamine, but more slowly | Thiobacillus thioparus | L-glutamate + NADP+ | - |
? | |
1.4.1.13 | L-glutamine + 2-oxoglutarate + NADPH + H+ | NH3-dependent activity is increased approximately 5-fold in apoglutamate synthase lacking flavin and non-heme iron | Thiobacillus thioparus | L-glutamate + NADP+ | - |
? | |
1.4.1.13 | additional information | alpha-ketoglutarate can not be replaced with pyruvate or oxalacetate | Thiobacillus thioparus | ? | - |
? | |
1.4.1.13 | NH3 + 2-oxoglutarate + NADPH + H+ | ammonia activity with 100 mM NH4Cl is about 6% of the glutamine activity with 5 mM L-glutamine | Thiobacillus thioparus | L-glutamate + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.1.13 | ? | x * 72000 + x * 94000 | Thiobacillus thioparus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.1.13 | -20 | - |
complete loss of activity after 10 days | Thiobacillus thioparus |
1.4.1.13 | 4 | - |
unprotected enzyme loses 90% of activity in 48 hours, protected enzyme, addition of 5 mM EDTA and 5 mM 2-mercaptoethanol or 1 mM DTT retains 80 to 90% of the activity after 48 hours | Thiobacillus thioparus |
1.4.1.13 | 100 | - |
30 s, complete loss of activity | Thiobacillus thioparus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.1.13 | 7.3 | 7.8 | - |
Thiobacillus thioparus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.13 | flavin | flavoenzyme | Thiobacillus thioparus | |
1.4.1.13 | NADH | no activity with NADH | Thiobacillus thioparus | |
1.4.1.13 | NADPH | - |
Thiobacillus thioparus |