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Literature summary extracted from

  • Griffin, M.; Kitcher, J.P.; Trudgill, P.W.
    Some studies on the purified 2-furoyl-coenzyme A hydroxylase from Pseudomonas putida F2 (1971), Biochem. J., 125, 19.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.99.8 0.02 0.05 2-Furoyl-CoA
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.99.8 900000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.99.8 2-furoyl-CoA + H2O + acceptor Pseudomonas putida
-
 S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
 ?
1.3.99.8 2-furoyl-CoA + H2O + acceptor Pseudomonas putida F2
-
 S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.8 Pseudomonas putida
-
-
-
1.3.99.8 Pseudomonas putida F2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.99.8 single absorption peak at 273 nm Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.8 2-furoyl-CoA + H2O + acceptor
-
 Pseudomonas putida S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
 ?
1.3.99.8 2-furoyl-CoA + H2O + acceptor acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida Pseudomonas putida S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
 ?
1.3.99.8 2-furoyl-CoA + H2O + acceptor
-
 Pseudomonas putida F2 S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
 ?
1.3.99.8 2-furoyl-CoA + H2O + acceptor acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida Pseudomonas putida F2 S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.99.8 FAD no flavin cofactor Pseudomonas putida