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Literature summary extracted from

  • Nagel, M.; Koenig, K.; Andreesen, J.R.
    Bactopterin as component of eubacterial dehydrogenases involved in hydroxylation reactions initiating the degradation of nicotine, nicotinate, and 2-furancarboxylate (1989), FEMS Microbiol. Lett., 60, 323-326.
No PubMed abstract available

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.99.8 Molybdenum
-
 Pseudomonas putida
1.5.99.4 Fe2+ four iron groups per mol Pseudarthrobacter oxydans
1.5.99.4 Molybdenum one molecule per mol Pseudarthrobacter oxydans
1.5.99.4 additional information two labile sulfur groups per mol Pseudarthrobacter oxydans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.99.8 2-furoyl-CoA + H2O + acceptor Pseudomonas putida
-
 S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
 ?
1.3.99.8 2-furoyl-CoA + H2O + acceptor Pseudomonas putida Fu1
-
 S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
 ?
1.17.3.3 additional information Neobacillus niacini catalyzes the second step in the degradation of nicotinate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.8 Pseudomonas putida
-
-
-
1.3.99.8 Pseudomonas putida Fu1
-
-
-
1.5.99.4 Pseudarthrobacter oxydans
-
 P-34 DSM 419
-
1.17.3.3 Neobacillus niacini
-
 strain DSM 2923
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.99.4 ammonium sulfate precipitation, ion-exchange, gel filtration Pseudarthrobacter oxydans
1.17.3.3 free of nicotinate dehydrogenase Neobacillus niacini

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.8 2-furoyl-CoA + H2O + acceptor
-
 Pseudomonas putida S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
 ?
1.3.99.8 2-furoyl-CoA + H2O + acceptor acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida Pseudomonas putida S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
 ?
1.3.99.8 2-furoyl-CoA + H2O + acceptor
-
 Pseudomonas putida Fu1 S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
 ?
1.3.99.8 2-furoyl-CoA + H2O + acceptor acceptors: nitro blue tetrazolium, methylene blue, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, membrane fraction of Pseudomonas putida Pseudomonas putida Fu1 S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor
-
 ?
1.5.99.4 nicotine + acceptor + H2O molybdate is required Pseudarthrobacter oxydans 6-hydroxynicotine + reduced acceptor
-
 ?
1.17.3.3 6-hydroxynicotinate + H2O + O2
-
 Neobacillus niacini 2,6-dihydroxynicotinate + H2O2
-
 ?
1.17.3.3 additional information catalyzes the second step in the degradation of nicotinate Neobacillus niacini ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.99.8 Bactopterin
-
 Pseudomonas putida
1.3.99.8 FAD
-
 Pseudomonas putida
1.5.99.4 FAD one mol per mol Pseudarthrobacter oxydans
1.5.99.4 pterin
-
 Pseudarthrobacter oxydans
1.17.3.3 Bactopterin component of the molybdenum cofactor Neobacillus niacini
1.17.3.3 FAD contains FAD as cofactor Neobacillus niacini
1.17.3.3 molybdenum cofactor with bactopterin as component Neobacillus niacini