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Literature summary extracted from
- Purification and characterization of the 3-ketosteroid-DELTA1-dehydrogenase of Arthrobacter simplex produced in Streptomyces lividans (1995), J. Biochem., 117, 1043-1049.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.99.4 | AgNO3 | - |
Pimelobacter simplex |  |
| 1.3.99.4 | CuCl2 | - |
Pimelobacter simplex | |
| 1.3.99.4 | HgCl2 | - |
Pimelobacter simplex | |
| 1.3.99.4 | additional information | not sensitive to carbonyl reagents and little sensitivity to metal chelating agents | Pimelobacter simplex | |
| 1.3.99.4 | p-chloromercuribenzoate | - |
Pimelobacter simplex | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.3.99.4 | 0.071 | - |
4-androstene-3,17-dione | - |
Pimelobacter simplex | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.3.99.4 | 55000 | - |
SDS-PAGE, gel filtration | Pimelobacter simplex |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 1.3.99.4 | Glycerol | 10%, stabilization | Pimelobacter simplex |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.3.99.4 | Pimelobacter simplex | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.3.99.4 | - |
Pimelobacter simplex |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.3.99.4 | 4°C, 10% glycerol, at least 3 months stable | Pimelobacter simplex |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.99.4 | 4-androstene-3,11,17-trione + acceptor | - |
Pimelobacter simplex | 1,4-androstene-3,11,17-trione + reduced acceptor | - |
? | |
| 1.3.99.4 | 4-androstene-3,17-dione + acceptor | - |
Pimelobacter simplex | 1,4-androstene-3,17-dione + reduced acceptor | - |
? | |
| 1.3.99.4 | 4-pregnene-3,20-dione + acceptor | - |
Pimelobacter simplex | 1,4-pregnene-3,20 dione + reduced acceptor | - |
? | |
| 1.3.99.4 | cortisone + acceptor | - |
Pimelobacter simplex | 1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced acceptor | - |
? | |
| 1.3.99.4 | testosterone + acceptor | - |
Pimelobacter simplex | 17beta-hydroxyandrost-1,4-diene-3-one + reduced acceptor | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.3.99.4 | monomer | 1 * 55000, SDS-PAGE, PAGE, gel filtration, N-terminal sequence | Pimelobacter simplex |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.3.99.4 | 37 | - |
assay at | Pimelobacter simplex |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.3.99.4 | 15 | 40 | maximum at 40°C, enzyme stable below 40°C | Pimelobacter simplex |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.3.99.4 | 10 | - |
substrate 4-androstene-3,17-dione, buffer 0.1 M glycine-NaOH | Pimelobacter simplex |
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