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Literature summary extracted from

  • Eder, M.; Krautle, F.; Dong, Y.; Vock, P.; Kieweg, V.; Kim, J.J.; Strauss, A.W.; Ghisla, S.
    Characterization of human and pig kidney long-chain-acyl-CoA dehydrogenases and their role in beta-oxidation (1997), Eur. J. Biochem., 245, 600-607.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.8.8 expression in Escherichia coli, strain BL21(DE) Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.8.8 2-octynoyl-CoA 90% inhibition at 5 mol inhibitor/mol enzyme Homo sapiens
1.3.8.8 2-octynoyl-CoA 65% inhibition at 5 mol inhibitor/mol enzyme Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.8.8 0.0015
-
 dodecanoyl-CoA
-
 Sus scrofa
1.3.8.8 0.0022
-
 decanoyl-CoA
-
 Sus scrofa
1.3.8.8 0.0023
-
 tetradecanoyl-CoA
-
 Sus scrofa
1.3.8.8 0.0033
-
 octanoyl-CoA
-
 Sus scrofa
1.3.8.8 0.0036
-
 palmitoyl-CoA
-
 Sus scrofa
1.3.8.8 0.0037
-
 stearoyl-CoA
-
 Sus scrofa
1.3.8.8 0.0063
-
 hexanoyl-CoA
-
 Sus scrofa
1.3.8.8 0.007
-
 dodecanoyl-CoA
-
 Homo sapiens
1.3.8.8 0.008
-
 octanoyl-CoA
-
 Homo sapiens
1.3.8.8 0.008
-
 stearoyl-CoA
-
 Homo sapiens
1.3.8.8 0.009
-
 electron-transfer flavoprotein saturating dodecanoyl-CoA Sus scrofa
1.3.8.8 0.01
-
 decanoyl-CoA
-
 Homo sapiens
1.3.8.8 0.01
-
 tetradecanoyl-CoA
-
 Homo sapiens
1.3.8.8 0.012
-
 electron-transfer flavoprotein saturating dodecanoyl-CoA, measured with ETF from pig kidney Homo sapiens
1.3.8.8 0.014
-
 palmitoyl-CoA
-
 Homo sapiens
1.3.8.8 0.029
-
 hexanoyl-CoA
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.8.8 44000
-
 x * 44000, SDS-PAGE Homo sapiens
1.3.8.8 44000
-
 x * 44000, SDS-PAGE Sus scrofa
1.3.8.8 180000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.7 Homo sapiens
-
-
-
1.3.8.8 Homo sapiens
-
-
-
1.3.8.8 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.8.8 40-80% ammonium sulfate precipitation, Q-Sepharose I, Q-Sepharose II, hydroxyapatite Homo sapiens
1.3.8.8 Q-Sepharose, hydroxyapatite I, hydroxyapatite II Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.8.8 kidney
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.8.8 15.27
-
 purified enzyme, decanoyl-CoA Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.7 dodecanoyl-CoA + electron-transfer flavoprotein
-
 Homo sapiens + trans-dodec-2-enoyl-CoA reduced electron-transfer flavoprotein
-
 r
1.3.8.8 decanoyl-CoA + electron-transfer flavoprotein
-
 Homo sapiens 2-decenoyl-CoA + reduced electron-transfer flavoprotein
-
 ?
1.3.8.8 decanoyl-CoA + electron-transfer flavoprotein
-
 Sus scrofa 2-decenoyl-CoA + reduced electron-transfer flavoprotein
-
 ?
1.3.8.8 dodecanoyl-CoA + electron-transfer flavoprotein
-
 Homo sapiens 2-dodecenoyl-CoA + reduced electron-transfer flavoprotein
-
 ?
1.3.8.8 dodecanoyl-CoA + electron-transfer flavoprotein
-
 Sus scrofa 2-dodecenoyl-CoA + reduced electron-transfer flavoprotein
-
 ?
1.3.8.8 hexanoyl-CoA + electron-transfer flavoprotein
-
 Homo sapiens 2-hexenoyl-CoA + reduced electron-transfer flavoprotein
-
 ?
1.3.8.8 hexanoyl-CoA + electron-transfer flavoprotein
-
 Sus scrofa 2-hexenoyl-CoA + reduced electron-transfer flavoprotein
-
 ?
1.3.8.8 octanoyl-CoA + electron-transfer flavoprotein
-
 Homo sapiens 2-octenoyl-CoA + reduced electron-transfer flavoprotein
-
 ?
1.3.8.8 octanoyl-CoA + electron-transfer flavoprotein
-
 Sus scrofa 2-octenoyl-CoA + reduced electron-transfer flavoprotein
-
 ?
1.3.8.8 palmitoyl-CoA + electron-transfer flavoprotein
-
 Homo sapiens 2-hexadecenoyl-CoA + reduced electron-transfer flavoprotein
-
 ?
1.3.8.8 palmitoyl-CoA + electron-transfer flavoprotein
-
 Sus scrofa 2-hexadecenoyl-CoA + reduced electron-transfer flavoprotein
-
 ?
1.3.8.8 stearoyl-CoA + electron-transfer flavoprotein
-
 Homo sapiens 2-octadecenoyl-CoA + reduced electron-transfer flavoprotein
-
 ?
1.3.8.8 stearoyl-CoA + electron-transfer flavoprotein
-
 Sus scrofa 2-octadecenoyl-CoA + reduced electron-transfer flavoprotein
-
 ?
1.3.8.8 tetradecanoyl-CoA + electron-transfer flavoprotein
-
 Homo sapiens 2-tetradecenoyl-CoA + reduced electron-transfer flavoprotein
-
 ?
1.3.8.8 tetradecanoyl-CoA + electron-transfer flavoprotein
-
 Sus scrofa 2-tetradecenoyl-CoA + reduced electron-transfer flavoprotein
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.8.8 ? x * 44000, SDS-PAGE Homo sapiens
1.3.8.8 ? x * 44000, SDS-PAGE Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.8.7 6
-
 tetradecanoyl-CoA
-
 Homo sapiens
1.3.8.7 7
-
 dodecanoyl-CoA
-
 Homo sapiens
1.3.8.7 11.7
-
 decanoyl-CoA
-
 Homo sapiens