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Literature summary extracted from

  • Thorpe, C.
    Acyl-CoA dehydrogenase from pig kidney (1981), Methods Enzymol., 71, 366-374.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.8.7 dialysis against distilled water at 4°C Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.8.7 diethyldicarbonate rapid inactivation Sus scrofa
1.3.8.7 iodoacetic acid S-carboxymethylated enzyme binds octanoyl-CoA but can no longer be reduced by it Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.8.7 42000
-
 4 * 42000, SDS-PAGE Sus scrofa
1.3.8.7 160000
-
 gel filtration Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.7 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.8.7
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.8.7 kidney
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.8.7 4
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
1.3.8.7 -20°C, 100 mM phosphate buffer, pH 7.6 Sus scrofa
1.3.8.7 4°C, ammonium sulfate suspension Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.7 acyl-CoA + acceptor electron acceptor: phenazine methosulfate Sus scrofa 2,3-dehydroacyl-CoA + reduced acceptor
-
 ?
1.3.8.7 acyl-CoA + acceptor intermediary electron acceptor: 2,6-dichloroindophenol Sus scrofa 2,3-dehydroacyl-CoA + reduced acceptor
-
 ?
1.3.8.7 acyl-CoA + acceptor final electron acceptor: 8-dimethylamino-2,3-benzophenoxazinium chloride, trivial name meldola's blue Sus scrofa 2,3-dehydroacyl-CoA + reduced acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.8.7 tetramer 4 * 42000, SDS-PAGE Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.8.7 3.25
-
 octanoyl-CoA phenazine methosulfate and 2,6-dichlorophenolindophenol as electron acceptors Sus scrofa