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Literature summary extracted from

  • Hara, M.; Morio, H.; Yazaki, K.; Tanaka, S.; Tabata, M.
    Separation and characterization of cytokinin-inducible (S)-tetrahydroberberine oxidases controlling berberine biosynthesis in Thalictrum minus cell cultures (1995), Phytochemistry, 38, 89-93.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.8 0.0021
-
 (S)-tetrahydroberberine isoenzyme I Thalictrum minus var. hypoleucum
1.3.3.8 0.0065
-
 (S)-tetrahydroberberine
-
 Coptis japonica
1.3.3.8 0.0106
-
 (S)-tetrahydroberberine isoenzyme II Thalictrum minus var. hypoleucum
1.3.3.8 0.0267
-
 (S)-tetrahydroberberine
-
 Berberis stolonifera

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.8 46000
-
 3 * 46000, isoenzyme II, gel filtration Thalictrum minus var. hypoleucum
1.3.3.8 58000
-
 3 * 58000 Coptis japonica
1.3.3.8 100000
-
 gel filtration Berberis stolonifera
1.3.3.8 145000
-
 isoenzyme II, gel filtration Thalictrum minus var. hypoleucum
1.3.3.8 155000
-
 gel filtration Coptis japonica
1.3.3.8 200000
-
 higher than, isoenzyme I, gel filtration Thalictrum minus var. hypoleucum

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.8 Berberis stolonifera
-
-
-
1.3.3.8 Coptis japonica
-
 Makino var. dissecta Nakai
-
1.3.3.8 Thalictrum dipterocarpum Franch.
-
-
-
1.3.3.8 Thalictrum flavum ssp. glaucum
-
-
-
1.3.3.8 Thalictrum minus var. hypoleucum
-
 native to Japan
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.8 copurification of three isoforms by ion-exchange Thalictrum minus var. hypoleucum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.3.8 cell culture
-
 Coptis japonica
-
1.3.3.8 cell culture
-
 Thalictrum minus var. hypoleucum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.8 (S)-tetrahydroberberine + 2 O2
-
 Berberis stolonifera berberine + 2 H2O2
-
 ?
1.3.3.8 (S)-tetrahydroberberine + 2 O2
-
 Coptis japonica berberine + 2 H2O2
-
 ?
1.3.3.8 (S)-tetrahydroberberine + 2 O2
-
 Thalictrum minus var. hypoleucum berberine + 2 H2O2
-
 ?
1.3.3.8 (S,R)-tetrahydrojatrorrhizine + O2
-
 Berberis stolonifera jatrorrhizine + H2O2
-
 ?
1.3.3.8 (S,R)-tetrahydrojatrorrhizine + O2
-
 Coptis japonica jatrorrhizine + H2O2
-
 ?
1.3.3.8 (S,R)-tetrahydrojatrorrhizine + O2
-
 Thalictrum flavum ssp. glaucum jatrorrhizine + H2O2
-
 ?
1.3.3.8 (S,R)-tetrahydrojatrorrhizine + O2
-
 Thalictrum dipterocarpum Franch. jatrorrhizine + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.3.8 trimer 3 * 58000 Coptis japonica
1.3.3.8 trimer 3 * 46000, isoenzyme II, gel filtration Thalictrum minus var. hypoleucum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.3.8 8.7
-
-
 Coptis japonica
1.3.3.8 8.9
-
-
 Berberis stolonifera
1.3.3.8 9
-
-
 Thalictrum minus var. hypoleucum