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Literature summary extracted from

  • Amann, M.; Nagakura, N.; Zenk, M.H.
    (S)-Tetrahydroprotoberberine oxidase the final enzyme in protoberberine biosynthesis (1984), Tetrahedron Lett., 25, 953-954.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.3.8 Morine 50% inhibition at 0.00018 mM Berberis wilsoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.8 0.0013
-
 (S)-Tetrahydrojatrorrhizine
-
 Berberis wilsoniae
1.3.3.8 0.0267
-
 (S)-canadine
-
 Berberis wilsoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.3.8 Fe2+ firmly bound to enzyme protein, involved in activity Papaver somniferum
1.3.3.8 Fe2+ firmly bound to enzyme protein, involved in activity Berberis wilsoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.8 100000
-
-
 Berberis wilsoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.8 (S)-tetrahydroberberine + 2 O2 Berberis wilsoniae 7,14-dehydroberberinium as intermediate, protoberberine biosynthesis protoberberine + 2 H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.8 Berberis wilsoniae
-
 var. subcaulialata
-
1.3.3.8 Papaver somniferum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.8
-
 Berberis wilsoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.8 (S)-canadine + O2
-
 Berberis wilsoniae ?
-
 ?
1.3.3.8 (S)-tetrahydroberberine + 2 O2
-
 Berberis wilsoniae berberine + 2 H2O2
-
 ?
1.3.3.8 (S)-tetrahydroberberine + 2 O2 7,14-dehydroberberinium as intermediate, protoberberine biosynthesis Berberis wilsoniae protoberberine + 2 H2O2
-
 ?
1.3.3.8 (S)-tetrahydrojatrorrhizine + O2
-
 Berberis wilsoniae ?
-
 ?
1.3.3.8 additional information specific for tetrahydroprotoberberines of the (S)-configuration, C-14 and N-7 of tetrahydroprotoberberines are oxidized Berberis wilsoniae ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.3.8 8.9
-
-
 Berberis wilsoniae

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.3.8 flavin
-
 Berberis wilsoniae