Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hooks, M.A.; Bode, K.; Couee, I.
    Higher-plant medium- and short-chain acyl-CoA oxidases: identification, purification and characterization of two novel enzymes of eukaryotic peroxisomal.beta-oxidation (1996), Biochem. J., 320, 607-614.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.6 0.002
-
 dodecanoyl-CoA MCOX Zea mays
1.3.3.6 0.006
-
 hexanoyl-CoA isozyme SCOX Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.3.6 peroxisome
-
 Zea mays 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.6 15000
-
 ? * 15000, isoform SCOX, labile multimeric structure, SDS-PAGE Zea mays
1.3.3.6 60000
-
 isoform SCOX, gel filtration Zea mays
1.3.3.6 62000
-
 isoform MCOX, gel filtration Zea mays

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.6 Zea mays
-
 medium-chain specific isoform, MCOX, and short-chain specific isoform, SCOX
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.6 both isoforms Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.3.6 seedling
-
 Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.3.6 0.375
-
 purified isozyme SCOX Zea mays
1.3.3.6 5.3
-
 highly purified isozyme MCOX Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.6 acyl-CoA + O2 isoform SCOX prefers C4-C8 substrates, isoform MCOX prefers C10-C14 substrates Zea mays trans-2,3-dehydroacyl-CoA + H2O2
-
 ?
1.3.3.6 dodecanoyl-CoA + O2
-
 Zea mays trans-dodec-2-enoyl-CoA + H2O2
-
 r
1.3.3.6 hexanoyl-CoA + O2
-
 Zea mays (2E)-hex-2-enoyl-CoA + H2O2
-
 ?
1.3.3.6 additional information structural and functional comparison of isoforms with each other and enzymes from other species, regulatory aspects Zea mays ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.3.6 ? ? * 15000, isoform SCOX, labile multimeric structure, SDS-PAGE Zea mays
1.3.3.6 monomer 1 * 62000, isoform MCOX, SDS-PAGE Zea mays

Synonyms

EC Number Synonyms Comment Organism
1.3.3.6 ACOX
-
 Zea mays
1.3.3.6 MCOX
-
 Zea mays
1.3.3.6 SCOX
-
 Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.3.6 8.3 8.6 isozyme SCOX Zea mays
1.3.3.6 8.6
-
 isozyme MCOX Zea mays

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.3.6 7.2 9.3 both isoforms Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.3.6 FAD flavoprotein with noncovalently bound FAD Zea mays