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Literature summary extracted from

  • Klemm, D.J.; Barton, L.L.
    Purification and properties of protoporphyrinogen oxidase from an anaerobic bacterium, Desulfovibrio gigas (1987), J. Bacteriol., 169, 5209-5215.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.3.4 iodoacetamide
-
 Megalodesulfovibrio gigas
1.3.3.4 N-ethylmaleimide
-
 Megalodesulfovibrio gigas

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.4 0.0021
-
 protoporphyrinogen-IX
-
 Megalodesulfovibrio gigas

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.3.4 membrane
-
 Megalodesulfovibrio gigas 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.4 12000
-
 2 * 12000 + 2 * 18500 + 2 * 57000 SDS-PAGE Megalodesulfovibrio gigas
1.3.3.4 18500
-
 2 * 12000 + 2 * 18500 + 2 * 57000 SDS-PAGE Megalodesulfovibrio gigas
1.3.3.4 57000
-
 2 * 12000 + 2 * 18500 + 2 * 57000 SDS-PAGE Megalodesulfovibrio gigas
1.3.3.4 148000
-
 gel filtration Megalodesulfovibrio gigas

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.4 protoporphyrinogen-IX + 3 O2 Megalodesulfovibrio gigas does not utilize O2 but 2,6-dichlorophenolindophenol as electron acceptor protoporphyrin-IX + 3 H2O2
-
 ?
1.3.3.4 protoporphyrinogen-IX + 3 O2 Megalodesulfovibrio gigas penultimate step of heme and chlorophyll biosynthesis protoporphyrin-IX + 3 H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.4 Megalodesulfovibrio gigas
-
 anaerobic, 2,6-dichlorophenolindophenol as electron acceptor
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.4
-
 Megalodesulfovibrio gigas

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.3.4 0.012
-
-
 Megalodesulfovibrio gigas

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.4 protoporphyrinogen-IX + 3 2,6-dichlorophenolindophenol anaerobic bacterium, electron acceptor other than O2 required, does not utilize NAD(P)+, FAD or FMN Megalodesulfovibrio gigas protoporphyrin-IX + 3 reduced 2,6-dichlorophenolindophenol
-
 ?
1.3.3.4 protoporphyrinogen-IX + 3 O2 similar enzyme from Desulfovibrio gigas does not use O2 as electron acceptor, but 2,6-dichlorophenol-indophenol Megalodesulfovibrio gigas protoporphyrin-IX + 3 H2O2
-
 ?
1.3.3.4 protoporphyrinogen-IX + 3 O2 does not utilize O2 but 2,6-dichlorophenolindophenol as electron acceptor Megalodesulfovibrio gigas protoporphyrin-IX + 3 H2O2
-
 ?
1.3.3.4 protoporphyrinogen-IX + 3 O2 penultimate step of heme and chlorophyll biosynthesis Megalodesulfovibrio gigas protoporphyrin-IX + 3 H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.3.4 hexamer 2 * 12000 + 2 * 18500 + 2 * 57000 SDS-PAGE Megalodesulfovibrio gigas

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.3.4 23
-
-
 Megalodesulfovibrio gigas

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.3.3.4 10 40 low activity below 10°C, no activity at 45°C Megalodesulfovibrio gigas

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.3.4 0.295
-
 protoporphyrinogen-IX
-
 Megalodesulfovibrio gigas

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.3.4 7 11
-
 Megalodesulfovibrio gigas

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.3.4 6.5 11.5
-
 Megalodesulfovibrio gigas