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Literature summary extracted from

  • Taton, M.; Husselstein, T.; Benveniste, P.; Rahier, A.
    Role of highly conserved residues in the reaction catalyzed by recombinant DELTA7-sterol-C5(6)-desaturase studied by site-directed mutagenesis (2000), Biochemistry, 39, 701-711.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.20
-
 Arabidopsis thaliana
1.14.19.20 expression in null mutant erg3 of Saccharomyces cerevisiae Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.14.19.20 G234A no activity Arabidopsis thaliana
1.14.19.20 G234A inactive mutant enzyme Arabidopsis thaliana
1.14.19.20 G234D 2% of wild-type activity Arabidopsis thaliana
1.14.19.20 G234D low activity Arabidopsis thaliana
1.14.19.20 H147E no activity Arabidopsis thaliana
1.14.19.20 H147E inactive mutant enzyme Arabidopsis thaliana
1.14.19.20 H147L no activity Arabidopsis thaliana
1.14.19.20 H147L inactive mutant enzyme Arabidopsis thaliana
1.14.19.20 H151E no activity Arabidopsis thaliana
1.14.19.20 H151E inactive mutant enzyme Arabidopsis thaliana
1.14.19.20 H151l no activity Arabidopsis thaliana
1.14.19.20 H151l inactive mutant enzyme Arabidopsis thaliana
1.14.19.20 H161E no activity Arabidopsis thaliana
1.14.19.20 H161E inactive mutant enzyme Arabidopsis thaliana
1.14.19.20 H161L no activity Arabidopsis thaliana
1.14.19.20 H161L inactive mutant enzyme Arabidopsis thaliana
1.14.19.20 H164L no activity Arabidopsis thaliana
1.14.19.20 H164L inactive mutant enzyme Arabidopsis thaliana
1.14.19.20 H165L no activity Arabidopsis thaliana
1.14.19.20 H165L inactive mutant enzyme Arabidopsis thaliana
1.14.19.20 H203L reduced activity Arabidopsis thaliana
1.14.19.20 H203L 13% of wild-type activity Arabidopsis thaliana
1.14.19.20 H222E reduced activity Arabidopsis thaliana
1.14.19.20 H222E 6% of wild-type activity Arabidopsis thaliana
1.14.19.20 H222L no activity Arabidopsis thaliana
1.14.19.20 H222L inactive mutant enzyme Arabidopsis thaliana
1.14.19.20 H238E no activity Arabidopsis thaliana
1.14.19.20 H238E inactive mutant enzyme Arabidopsis thaliana
1.14.19.20 H238L no activity Arabidopsis thaliana
1.14.19.20 H238L inactive mutant enzyme Arabidopsis thaliana
1.14.19.20 H241L no activity Arabidopsis thaliana
1.14.19.20 H241L inactive mutant enzyme Arabidopsis thaliana
1.14.19.20 H242L no activity Arabidopsis thaliana
1.14.19.20 H242L inactive mutant enzyme Arabidopsis thaliana
1.14.19.20 K115L 2times higher activity than wild-type Arabidopsis thaliana
1.14.19.20 K115L Km-value for 5alpha-cholest-7-en-3beta-ol is 2.8fold higher than that of wild-type activity Arabidopsis thaliana
1.14.19.20 P175A wild-type activity Arabidopsis thaliana
1.14.19.20 P175A as active as wild-type enzyme Arabidopsis thaliana
1.14.19.20 P175V 4times higher activity than wild-type Arabidopsis thaliana
1.14.19.20 P175V Km-value for 5alpha-cholest-7-en-3beta-ol is 2.7fold higher than that of wild-type activity Arabidopsis thaliana
1.14.19.20 P201A 25% of wild-type activity Arabidopsis thaliana
1.14.19.20 P201A 20% of wild-type activity Arabidopsis thaliana
1.14.19.20 T114I wild-type activity Arabidopsis thaliana
1.14.19.20 T114I | Km-value for 5alpha-cholest-7-en-3beta-ol is 7.5fold higher than that of wild-type activity Arabidopsis thaliana
1.14.19.20 T114S 28times higher activity than wild-type Arabidopsis thaliana
1.14.19.20 T114S Km-value for 5alpha-cholest-7-en-3beta-ol is 7.6fold higher than that of wild-type activity Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.19.20 CN- 0.003 mM, 50% inhibition of recombinant enzyme Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.19.20 0.032
-
 5alpha-Cholest-7-en-3beta-ol
-
 Arabidopsis thaliana
1.14.19.20 0.032
-
 5alpha-Cholest-7-en-3beta-ol 30°C, wild-type enzyme Arabidopsis thaliana
1.14.19.20 0.07
-
 5alpha-Cholest-7-en-3beta-ol P175a mutant enzyme Arabidopsis thaliana
1.14.19.20 0.07
-
 5alpha-Cholest-7-en-3beta-ol 30°C, mutant enzyme P175A Arabidopsis thaliana
1.14.19.20 0.086
-
 5alpha-Cholest-7-en-3beta-ol P175V mutant enzyme Arabidopsis thaliana
1.14.19.20 0.086
-
 5alpha-Cholest-7-en-3beta-ol 30°C, mutant enzyme P175V Arabidopsis thaliana
1.14.19.20 0.09
-
 5alpha-Cholest-7-en-3beta-ol K115L mutant enzyme Arabidopsis thaliana
1.14.19.20 0.09
-
 5alpha-Cholest-7-en-3beta-ol 30°C, mutant enzyme K115L Arabidopsis thaliana
1.14.19.20 0.24
-
 5alpha-Cholest-7-en-3beta-ol T114I mutant enzyme Arabidopsis thaliana
1.14.19.20 0.24
-
 5alpha-Cholest-7-en-3beta-ol 30°C, mutant enzyme T114I Arabidopsis thaliana
1.14.19.20 0.244
-
 5alpha-Cholest-7-en-3beta-ol T114S mutant enzyme Arabidopsis thaliana
1.14.19.20 0.244
-
 5alpha-Cholest-7-en-3beta-ol 30°C, mutant enzyme T114S Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.20 Arabidopsis thaliana Q39208
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.20 5alpha-cholest-7-en-3beta-ol + ferrocytochrmoe b5 + H+ + O2 NADH is more efficient than NADPH Arabidopsis thaliana cholesta-5,7-dien-3beta-ol + ferricytochrome b5 + H2O
-
 ?
1.14.19.20 5alpha-cholest-7-en-3beta-ol + ferrocytochrome b5 + H+ + O2 NADH is more efficient than NADPH Arabidopsis thaliana cholesta-5,7-dien-3beta-ol + ferricytochrome b5 + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.20 5-DES
-
 Arabidopsis thaliana
1.14.19.20 DELTA7-sterol 5-desaturase
-
 Arabidopsis thaliana
1.14.19.20 DELTA7-sterol DELTA5-dehydrogenase
-
 Arabidopsis thaliana
1.14.19.20 DELTA7-sterol-C5(6)-desaturase
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.20 NAD(P)H
-
 Arabidopsis thaliana
1.14.19.20 NADH NADH is more efficient than NADPH Arabidopsis thaliana
1.14.19.20 NADPH NADH is more efficient than NADPH Arabidopsis thaliana