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Literature summary extracted from

  • Copeland, R.A.; Davis, J.P.; Dowling, R.L.; Lombardo, D.; Murphy, K.B.; Patterson, T.A.
    Recombinant human dihydroorotate dehydrogenase: expression, purification, and characterization of a catalytically functional truncated enzyme (1995), Arch. Biochem. Biophys., 323, 79-86.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.3.5.2 medicine great interest in inhibitors as potential therapeutic agents for the treatment of diseases involving aberrant cell proliferation Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.5.2
-
Homo sapiens
1.3.5.2 expressed in Escherichia coli DH5alpha TAP330, lacking the endogenous gene for the bacterial dihydroorotate dehydrogenase, pyrimidine auxotroph Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.5.2 brequinar sodium potent, noncompetitive inhibitor Homo sapiens
1.3.5.2 diethyldicarbonate histidine-selective covalent modifier; histidine-selective modifying agent, dose-dependent Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.5.2 0.0155
-
S-dihydroorotate
-
Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.5.2 membrane
-
Homo sapiens 16020
-
1.3.5.2 mitochondrion inner membrane Homo sapiens 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.5.2 40000
-
x * 40000, recombinant enzyme, SDS-PAGE, x * 50000, native full-length enzyme, SDS-PAGE Homo sapiens
1.3.5.2 40000
-
? * 40000 Homo sapiens
1.3.5.2 50000
-
x * 40000, recombinant enzyme, SDS-PAGE, x * 50000, native full-length enzyme, SDS-PAGE Homo sapiens
1.3.5.2 120000
-
gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.5.2 (S)-dihydroorotate + ubiquinone Homo sapiens fourth step in synthesis of uridine 5'-monophosphate orotate + ubiquinol
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.2 Homo sapiens
-
-
-
1.3.5.2 Homo sapiens
-
truncated recombinant protein
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.5.2 ammonium sulfate precipitation, affinity chromatography, ion-exchange chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.5.2 liver
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.5.2 1.49
-
-
Homo sapiens

Storage Stability

EC Number Storage Stability Organism
1.3.5.2 -70°C, flash frozen Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.2 (S)-dihydroorotate + ubiquinone
-
Homo sapiens orotate + ubiquinol
-
?
1.3.5.2 (S)-dihydroorotate + ubiquinone fourth step in synthesis of uridine 5'-monophosphate Homo sapiens orotate + ubiquinol
-
?

Subunits

EC Number Subunits Comment Organism
1.3.5.2 ? x * 40000, recombinant enzyme, SDS-PAGE, x * 50000, native full-length enzyme, SDS-PAGE Homo sapiens
1.3.5.2 ? ? * 40000 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.3.5.2 DHODase
-
Homo sapiens
1.3.5.2 L-5,6-dihydroorotate:ubiquinone exidoreductase
-
Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.5.2 8
-
full-length native enzyme Homo sapiens
1.3.5.2 8.5
-
recombinant enzyme Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.5.2 additional information does not contain a flavin cofactor Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.5.2 0.0000232
-
brequinar sodium full-length native enzyme Homo sapiens
1.3.5.2 0.0000265
-
brequinar sodium recombinant enzyme Homo sapiens