EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.3.5.2 | S175A | less than 0.01% of activity of the wild-type enzyme | Escherichia coli |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.3.5.2 | membrane | - |
Escherichia coli | 16020 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.5.2 | (S)-dihydroorotate + acceptor | Escherichia coli | fourth step in synthesis of pyrimidine nucleotides | orotate + reduced acceptor | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.5.2 | Escherichia coli | - |
- |
- |
1.3.5.2 | Escherichia coli | - |
family 2 enzyme | - |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.3.5.2 | (S)-dihydroorotate + a quinone = orotate + a quinol | existence of 3 families differing in their selectivity for oxidizing substrates: 1A are soluble, containing FMN and use fumarate, 1B are soluble, one subunit contains an iron-sulfur center, FAD and reduces NAD+, family 2 enzymes are membrane-bound, contain FMN and are oxidized by ubiquinone. Mechanism consists of 3 reaction phases. Different binding-mechanisms for enzyme the reaction-steps are suggested | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.5.2 | (S)-dihydroorotate + acceptor | uses ubiquinone as electron acceptor | Escherichia coli | orotate + reduced acceptor | - |
? | |
1.3.5.2 | (S)-dihydroorotate + acceptor | fourth step in synthesis of pyrimidine nucleotides | Escherichia coli | orotate + reduced acceptor | - |
? | |
1.3.5.2 | (S)-dihydroorotate + ubiquinone | reaction studied with menadione, O2 and ferricyanide | Escherichia coli | orotate + ubiquinol | - |
? | |
1.3.5.2 | dihydroorotate + acceptor | reaction intermediates, mechanism, pH-dependence of reaction | Escherichia coli | orotate + reduced acceptor | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.5.2 | monomer | - |
Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.5.2 | DHOD | - |
Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.5.2 | 19.7 | - |
S-dihydroorotate | pH 8.5, 4°C, with menadione | Escherichia coli |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.3.5.2 | 6 | 12.7 | - |
Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.5.2 | FMN | - |
Escherichia coli |