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Literature summary extracted from

  • Ostergaard, J.; Persiau, G.; Davey, M.W.; Bauw, G.; Van Montagu, M.
    Isolation of a cDNA coding for L-galactono-gamma-lactone dehydrogenase, an enzyme involved in the biosynthesis of ascorbic acid in plants. Purification, characterization, cDNA cloning, and expression in yeast (1997), J. Biol. Chem., 272, 30009-30016.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.2.3 expression in Saccharomyces cerevisiae Brassica oleracea var. botrytis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.2.3 iodoacetic acid
-
 Brassica oleracea var. botrytis
1.3.2.3 L-galactono-gamma-lactone substrate inhibition at concentration above 32.6 mM Brassica oleracea var. botrytis
1.3.2.3 additional information no inhibition with riboflavin and lycorine Brassica oleracea var. botrytis
1.3.2.3 N-ethylmaleimide
-
 Brassica oleracea var. botrytis
1.3.2.3 p-hydroxymercuribenzoic acid
-
 Brassica oleracea var. botrytis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.2.3 3.3
-
 L-galactono-gamma-lactone
-
 Brassica oleracea var. botrytis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.2.3 mitochondrion
-
 Brassica oleracea var. botrytis 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.2.3 additional information Mg2+, Ca2+ and Sr2+ have no effect on activity Brassica oleracea var. botrytis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.2.3 56000
-
 gel filtration Brassica oleracea var. botrytis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.2.3 L-galactono-1,4-lactone + 2 ferricytochrome c Brassica oleracea var. botrytis terminal step in L-ascorbic acid biosynthesis L-ascorbate + 2 ferrocytochrome c + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.2.3 Brassica oleracea var. botrytis O47881
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.2.3
-
 Brassica oleracea var. botrytis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.2.3 3
-
 extract of yeast expressing the recombinant enzyme Brassica oleracea var. botrytis
1.3.2.3 50800
-
 purified enzyme Brassica oleracea var. botrytis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.2.3 L-galactono-1,4-lactone + 2 ferricytochrome c terminal step in L-ascorbic acid biosynthesis Brassica oleracea var. botrytis L-ascorbate + 2 ferrocytochrome c + 2 H+
-
 ?
1.3.2.3 L-galactono-1,4-lactone + ferricytochrome c specific for cytochrome c as electron acceptor Brassica oleracea var. botrytis L-ascorbate + ferrocytochrome c
-
 ?
1.3.2.3 L-galactono-1,4-lactone + ferricytochrome c native and recombinant from yeast Brassica oleracea var. botrytis L-ascorbate + ferrocytochrome c
-
 ?
1.3.2.3 L-galactono-1,4-lactone + ferricytochrome c specific for L-galactono-gamma-lactone Brassica oleracea var. botrytis L-ascorbate + ferrocytochrome c
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.2.3 monomer 1 * 56000, SDS-PAGE Brassica oleracea var. botrytis

Synonyms

EC Number Synonyms Comment Organism
1.3.2.3 GLDase
-
 Brassica oleracea var. botrytis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.2.3 22
-
 assay at Brassica oleracea var. botrytis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.2.3 8 8.5
-
 Brassica oleracea var. botrytis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.2.3 6 8.8
-
 Brassica oleracea var. botrytis

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.2.3 flavin not Brassica oleracea var. botrytis