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Literature summary extracted from

  • Slabas, A.R.; Sidebottom, C.M.; Hellyer, A.; Kessell, R.M.J.; Tombs, M.P.
    Induction, purification and characterization of NADH-specific enoyl acyl carrier protein reductase from developing seeds of oil seed rape (Brassica napus) (1986), Biochim. Biophys. Acta, 877, 271-280.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.9 iodoacetamide no inhibition at 10 mM Brassica napus
1.3.1.9 N-ethylmaleimide no inhibition at 1 mM, 97% inhibition at 10 mM, inhibition can be partially reversed by dithiothreitol Brassica napus
1.3.1.9 p-chloromercuribenzoate complete inhibition at 0.1 mM, inhibition can be prevented by preincubation with crotonyl-CoA Brassica napus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.9 0.001
-
 crotonyl-[acyl-carrier protein] Km is 0.001mM or less Brassica napus
1.3.1.9 0.0076
-
 NADH
-
 Brassica napus
1.3.1.9 0.178
-
 crotonyl-CoA
-
 Brassica napus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.9 33600
-
 alpha2beta2, 2 *: 34800, 2 * 33600, SDS-PAGE Brassica napus
1.3.1.9 140000
-
 gel filtration Brassica napus

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.9 Brassica napus
-
 rape
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.9 homogeneity Brassica napus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.9 seed
-
 Brassica napus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.9 200
-
-
 Brassica napus

Storage Stability

EC Number Storage Stability Organism
1.3.1.9 -70°C, at least 3 months, survives only 1 freeze thaw cycle Brassica napus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.9 crotonyl-CoA + NADH
-
 Brassica napus butyryl-CoA + NAD+
-
 ?
1.3.1.9 crotonyl-[acyl-carrier protein] + NADH
-
 Brassica napus butyryl-[acyl-carrier protein] + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.9 tetramer alpha2beta2, 2 *: 34800, 2 * 33600, SDS-PAGE Brassica napus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.9 6
-
 in 3-(N-morpholino)propane sulfonate buffer Brassica napus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.9 4.5 8.5
-
 Brassica napus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.9 NADH absolute specificity Brassica napus