BRENDA - Enzyme Database

Purification of a 15-ketoprostaglandin DELTA13-reductase from rat liver and its ability to reduce the double bond of xenobiotics

Kitamura, S.; Katsura, H.; Tatsumi, K.; Biochem. Mol. Biol. Int. 30, 839-847 (1993)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.1.48
dicoumarol
0.1 mM, complete inhibition
Rattus norvegicus
1.3.1.48
indomethacin
0.1 mM, 95% inhibition
Rattus norvegicus
1.3.1.48
p-chloromercuribenzoate
0.1 mM, 97% inhibition
Rattus norvegicus
1.3.1.48
quercitrin
95% inhibition
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.1.48
0.000213
-
15-ketoprostaglandin F2alpha
-
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.1.48
39500
-
1 * 39500, SDS-PAGE
Rattus norvegicus
1.3.1.48
42000
-
gel filtration
Rattus norvegicus
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.3.1.48
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.3.1.48
DEAE-cellulose, phenyl-toyopearl, blue-toyopearl, hydroxyapatite, liver enzyme
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.3.1.48
adrenal gland
-
Rattus norvegicus
-
1.3.1.48
brain
-
Rattus norvegicus
-
1.3.1.48
heart
-
Rattus norvegicus
-
1.3.1.48
kidney
-
Rattus norvegicus
-
1.3.1.48
liver
-
Rattus norvegicus
-
1.3.1.48
lung
-
Rattus norvegicus
-
1.3.1.48
pancreas
-
Rattus norvegicus
-
1.3.1.48
small intestine
-
Rattus norvegicus
-
1.3.1.48
spleen
-
Rattus norvegicus
-
1.3.1.48
testis
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.1.48
2.05
-
-
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.3.1.48
(5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NAD(P)H
-
390826
Rattus norvegicus
(5Z)-11alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+
-
390826
Rattus norvegicus
?
1.3.1.48
(Z)-3-[(5-methoxy-3-oxo-2(3H)-benzofuranylidene)-methyl]benzoic acid + NAD(P)H
-
390826
Rattus norvegicus
3-[(5-methoxy-3-oxo-2,3-dihydro-1-benzofuran-2-yl)methyl]benzoic acid + NAD(P)+
-
390826
Rattus norvegicus
?
1.3.1.48
1-(p-carboxyphenyl)-5-cyano-3-oxo-1-pentene + NAD(P)H
-
390826
Rattus norvegicus
1-(p-carboxyphenyl)-5-cyano-3-oxo-pentane + NAD(P)+
-
390826
Rattus norvegicus
?
1.3.1.48
11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NAD(P)H
-
390826
Rattus norvegicus
11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NAD(P)+
-
390826
Rattus norvegicus
?
1.3.1.48
15-ketoprostaglandin F1alpha + NAD(P)H
-
390826
Rattus norvegicus
?
-
-
-
?
1.3.1.48
15-ketoprostaglandin F2alpha + NADPH
-
390826
Rattus norvegicus
?
-
-
-
?
1.3.1.48
beta-ionone + NAD(P)H
-
390826
Rattus norvegicus
?
-
-
-
?
1.3.1.48
curcumin + NAD(P)H
-
390826
Rattus norvegicus
?
-
-
-
?
1.3.1.48
trans-2-(o-hydroxybenzylidene)acetophenone + NAD(P)H
-
390826
Rattus norvegicus
(o-hydroxybenzyl)acetophenone + NAD(P)+
-
390826
Rattus norvegicus
?
1.3.1.48
trans-2-benzylidenecyclohexan-2-one + NAD(P)H
-
390826
Rattus norvegicus
benzylcyclohexan-2-one + NAD(P)+
-
390826
Rattus norvegicus
?
1.3.1.48
trans-2-butenal + NAD(P)H
-
390826
Rattus norvegicus
butanal + NAD(P)+
-
390826
Rattus norvegicus
?
1.3.1.48
trans-2-hexenal + NAD(P)H
-
390826
Rattus norvegicus
hexanal + NAD(P)+
-
390826
Rattus norvegicus
?
1.3.1.48
trans-3-(2-furyl)acrolein + NAD(P)H
-
390826
Rattus norvegicus
2-furylpropanal + NAD(P)+
-
390826
Rattus norvegicus
?
1.3.1.48
trans-4-furyl-3-buten-2-one + NAD(P)H
-
390826
Rattus norvegicus
4-furyl-butane-2-one + NAD(P)+
-
390826
Rattus norvegicus
?
1.3.1.48
trans-benzylideneacetone + NAD(P)H
-
390826
Rattus norvegicus
benzylacetone + NAD(P)+
-
390826
Rattus norvegicus
?
1.3.1.48
trans-benzylideneacetophenone + NAD(P)H
-
390826
Rattus norvegicus
benzylacetophenone + NAD(P)+
-
390826
Rattus norvegicus
?
1.3.1.48
trans-cinnamaldehyde + NAD(P)H
-
390826
Rattus norvegicus
?
-
-
-
?
1.3.1.48
trans-cinnamic acid ethyl ester + NAD(P)H
-
390826
Rattus norvegicus
?
-
-
-
?
1.3.1.48
trans-cinnamoyl thiophene + NAD(P)H
-
390826
Rattus norvegicus
?
-
-
-
?
1.3.1.48
trans-methylvinylketone + NAD(P)H
-
390826
Rattus norvegicus
butane-2-one + NAD(P)+
-
390826
Rattus norvegicus
?
1.3.1.48
trans-phenyl-1-propenyl ketone + NAD(P)H
-
390826
Rattus norvegicus
phenylpropane-1-one + NAD+
-
390826
Rattus norvegicus
?
1.3.1.48
trans-trans-2,4-hexadienal + NAD(P)H
-
390826
Rattus norvegicus
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.3.1.48
monomer
1 * 39500, SDS-PAGE
Rattus norvegicus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.3.1.48
6.5
8
-
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.3.1.48
NAD(P)H
in crude extracts: NADH is 30% as effective as NADPH, in purified enzyme: NADH is 10% as effectic as NADPH
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.3.1.48
NAD(P)H
in crude extracts: NADH is 30% as effective as NADPH, in purified enzyme: NADH is 10% as effectic as NADPH
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.1.48
dicoumarol
0.1 mM, complete inhibition
Rattus norvegicus
1.3.1.48
indomethacin
0.1 mM, 95% inhibition
Rattus norvegicus
1.3.1.48
p-chloromercuribenzoate
0.1 mM, 97% inhibition
Rattus norvegicus
1.3.1.48
quercitrin
95% inhibition
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.1.48
0.000213
-
15-ketoprostaglandin F2alpha
-
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.1.48
39500
-
1 * 39500, SDS-PAGE
Rattus norvegicus
1.3.1.48
42000
-
gel filtration
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.1.48
DEAE-cellulose, phenyl-toyopearl, blue-toyopearl, hydroxyapatite, liver enzyme
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.3.1.48
adrenal gland
-
Rattus norvegicus
-
1.3.1.48
brain
-
Rattus norvegicus
-
1.3.1.48
heart
-
Rattus norvegicus
-
1.3.1.48
kidney
-
Rattus norvegicus
-
1.3.1.48
liver
-
Rattus norvegicus
-
1.3.1.48
lung
-
Rattus norvegicus
-
1.3.1.48
pancreas
-
Rattus norvegicus
-
1.3.1.48
small intestine
-
Rattus norvegicus
-
1.3.1.48
spleen
-
Rattus norvegicus
-
1.3.1.48
testis
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.1.48
2.05
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.3.1.48
(5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NAD(P)H
-
390826
Rattus norvegicus
(5Z)-11alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+
-
390826
Rattus norvegicus
?
1.3.1.48
(Z)-3-[(5-methoxy-3-oxo-2(3H)-benzofuranylidene)-methyl]benzoic acid + NAD(P)H
-
390826
Rattus norvegicus
3-[(5-methoxy-3-oxo-2,3-dihydro-1-benzofuran-2-yl)methyl]benzoic acid + NAD(P)+
-
390826
Rattus norvegicus
?
1.3.1.48
1-(p-carboxyphenyl)-5-cyano-3-oxo-1-pentene + NAD(P)H
-
390826
Rattus norvegicus
1-(p-carboxyphenyl)-5-cyano-3-oxo-pentane + NAD(P)+
-
390826
Rattus norvegicus
?
1.3.1.48
11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NAD(P)H
-
390826
Rattus norvegicus
11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NAD(P)+
-
390826
Rattus norvegicus
?
1.3.1.48
15-ketoprostaglandin F1alpha + NAD(P)H
-
390826
Rattus norvegicus
?
-
-
-
?
1.3.1.48
15-ketoprostaglandin F2alpha + NADPH
-
390826
Rattus norvegicus
?
-
-
-
?
1.3.1.48
beta-ionone + NAD(P)H
-
390826
Rattus norvegicus
?
-
-
-
?
1.3.1.48
curcumin + NAD(P)H
-
390826
Rattus norvegicus
?
-
-
-
?
1.3.1.48
trans-2-(o-hydroxybenzylidene)acetophenone + NAD(P)H
-
390826
Rattus norvegicus
(o-hydroxybenzyl)acetophenone + NAD(P)+
-
390826
Rattus norvegicus
?
1.3.1.48
trans-2-benzylidenecyclohexan-2-one + NAD(P)H
-
390826
Rattus norvegicus
benzylcyclohexan-2-one + NAD(P)+
-
390826
Rattus norvegicus
?
1.3.1.48
trans-2-butenal + NAD(P)H
-
390826
Rattus norvegicus
butanal + NAD(P)+
-
390826
Rattus norvegicus
?
1.3.1.48
trans-2-hexenal + NAD(P)H
-
390826
Rattus norvegicus
hexanal + NAD(P)+
-
390826
Rattus norvegicus
?
1.3.1.48
trans-3-(2-furyl)acrolein + NAD(P)H
-
390826
Rattus norvegicus
2-furylpropanal + NAD(P)+
-
390826
Rattus norvegicus
?
1.3.1.48
trans-4-furyl-3-buten-2-one + NAD(P)H
-
390826
Rattus norvegicus
4-furyl-butane-2-one + NAD(P)+
-
390826
Rattus norvegicus
?
1.3.1.48
trans-benzylideneacetone + NAD(P)H
-
390826
Rattus norvegicus
benzylacetone + NAD(P)+
-
390826
Rattus norvegicus
?
1.3.1.48
trans-benzylideneacetophenone + NAD(P)H
-
390826
Rattus norvegicus
benzylacetophenone + NAD(P)+
-
390826
Rattus norvegicus
?
1.3.1.48
trans-cinnamaldehyde + NAD(P)H
-
390826
Rattus norvegicus
?
-
-
-
?
1.3.1.48
trans-cinnamic acid ethyl ester + NAD(P)H
-
390826
Rattus norvegicus
?
-
-
-
?
1.3.1.48
trans-cinnamoyl thiophene + NAD(P)H
-
390826
Rattus norvegicus
?
-
-
-
?
1.3.1.48
trans-methylvinylketone + NAD(P)H
-
390826
Rattus norvegicus
butane-2-one + NAD(P)+
-
390826
Rattus norvegicus
?
1.3.1.48
trans-phenyl-1-propenyl ketone + NAD(P)H
-
390826
Rattus norvegicus
phenylpropane-1-one + NAD+
-
390826
Rattus norvegicus
?
1.3.1.48
trans-trans-2,4-hexadienal + NAD(P)H
-
390826
Rattus norvegicus
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.3.1.48
monomer
1 * 39500, SDS-PAGE
Rattus norvegicus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.3.1.48
6.5
8
-
Rattus norvegicus