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Literature summary extracted from

  • Shimakata, T.; Fujita, Y.; Kusaka, T.
    Involvement of one of two enoyl-CoA hydratases and enoyl-CoA reductase in the acetyl-CoA-dependent elongation of medium chain fatty acids by Mycobacterium smegmatis (1980), J. Biochem., 88, 1051-1058.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.44 additional information substrates with chain-length higher than C16, concentrations above 0.02 mM Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.44 0.021
-
 NADH
-
 Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.44 72000
-
 gel filtration Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.44 acyl-CoA + NAD+ Mycolicibacterium smegmatis fatty acid elongation trans-2,3-didehydroacyl-CoA + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.44 Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.44 partial Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.44 0.351
-
-
 Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.44 acyl-CoA + NAD+ fatty acid elongation Mycolicibacterium smegmatis trans-2,3-didehydroacyl-CoA + NADH
-
 ?
1.3.1.44 additional information inert to substrates with chain length below C8 Mycolicibacterium smegmatis ?
-
 ?
1.3.1.44 trans-2-decenoyl-CoA + NADH
-
 Mycolicibacterium smegmatis decanoyl-CoA + NAD+
-
 ?
1.3.1.44 trans-2-dodecenoyl-CoA + NADH
-
 Mycolicibacterium smegmatis dodecanoyl-CoA + NAD+
-
 ?
1.3.1.44 trans-2-hexadecenoyl-CoA + NADH
-
 Mycolicibacterium smegmatis hexadecanoyl-CoA + NAD+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.44 5 5.5
-
 Mycolicibacterium smegmatis

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.44 NADH sole electron donor Mycolicibacterium smegmatis