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Literature summary extracted from

  • Inui, H.; Miyatake, K.; Nakano, Y.; Kitaoka, S.
    Purification and some properties of short chain-length specific trans-2-enoyl-CoA reductase in mitochondria of Euglena gracilis (1986), J. Biochem., 100, 995-1000.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.44 Co2+ only after preincubation with cation Euglena gracilis
1.3.1.44 KCN protection by FAD Euglena gracilis
1.3.1.44 Mn2+ only after preincubation with cation Euglena gracilis
1.3.1.44 N-ethylmaleimide protection by FAD Euglena gracilis
1.3.1.44 Ni2+ only after preincubation with cation Euglena gracilis
1.3.1.44 p-chloromercuribenzoate protection by FAD Euglena gracilis
1.3.1.44 Zn2+ only after preincubation with cation Euglena gracilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.44 mitochondrion
-
 Euglena gracilis 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.44 15000
-
 1 * 15000 + 1 * 25000, SDS-PAGE Euglena gracilis
1.3.1.44 25000
-
 1 * 15000 + 1 * 25000, SDS-PAGE Euglena gracilis
1.3.1.44 39000
-
 gel filtration Euglena gracilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.44 acyl-CoA + NAD+ Euglena gracilis contributes to fatty acid synthesis beta-oxidation in mitochondria trans-2,3-didehydroacyl-CoA + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.44 Euglena gracilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.44
-
 Euglena gracilis

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.1.44 acyl-CoA + NAD+ = trans-didehydroacyl-CoA + NADH + H+ short chain-length specific Euglena gracilis
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.44 0.002
-
-
 Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.44 acyl-CoA + NAD+ contributes to fatty acid synthesis beta-oxidation in mitochondria Euglena gracilis trans-2,3-didehydroacyl-CoA + NADH
-
 ?
1.3.1.44 crotonyl-CoA + NADH negative cooperativity in the reaction with substrate Euglena gracilis butyryl-CoA + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.44 dimer 1 * 15000 + 1 * 25000, SDS-PAGE Euglena gracilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.44 30
-
 at pH 6.2 Euglena gracilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.44 6.2
-
-
 Euglena gracilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.44 5.2 7 less than 50% of maximal activity above pH 7.0 and below pH 5.2 Euglena gracilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.44 flavin FAD, riboflavin and FMN increase activity Euglena gracilis
1.3.1.44 NADH sole electron donor Euglena gracilis