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Literature summary extracted from

  • Xie, G.; Bonner, C.A.; Jensen, R.A.
    Cyclohexadienyl dehydrogenase from Pseudomonas stutzeri exemplifies a widespread type of tyrosine-pathway dehydrogenase in the TyrA protein family (2000), Comp. Biochem. Physiol. C, 125, 65-83.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.43 tyrAc gene, sequence analysis, overexpression of His-tagged protein in Escherichia coli Pseudomonas stutzeri

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.43 3,4-dihydroxyphenylalanine slight inhibition Pseudomonas stutzeri
1.3.1.43 4-amino-L-phenylalanine
-
 Pseudomonas stutzeri
1.3.1.43 4-hydroxybenzoate
-
 Pseudomonas stutzeri
1.3.1.43 4-hydroxyphenylacetate
-
 Pseudomonas stutzeri
1.3.1.43 4-hydroxyphenylpyruvate
-
 Pseudomonas stutzeri
1.3.1.43 D-tyrosine
-
 Pseudomonas stutzeri
1.3.1.43 DL-4-hydroxyphenyllactate
-
 Pseudomonas stutzeri
1.3.1.43 L-phenylalanine slight inhibition Pseudomonas stutzeri
1.3.1.43 L-tyrosine competitive inhibition Pseudomonas stutzeri
1.3.1.43 m-Fluoro-DL-tyrosine
-
 Pseudomonas stutzeri
1.3.1.43 additional information no inhibition with phenyllactate and benzoate Pseudomonas stutzeri
1.3.1.43 phenol
-
 Pseudomonas stutzeri
1.3.1.43 phenylpyruvate no inhibition Pseudomonas stutzeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.43 0.07
-
 prephenate
-
 Pseudomonas stutzeri
1.3.1.43 0.14
-
 NAD+
-
 Pseudomonas stutzeri
1.3.1.43 0.56
-
 L-arogenate
-
 Pseudomonas stutzeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.43 33000
-
 2 * 33000, recombinant His-tagged protein, forms a tetramer at high protein concentration, SDS-PAGE Pseudomonas stutzeri
1.3.1.43 66000
-
 dimer, gel filtration Pseudomonas stutzeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.43 L-arogenate + NAD+ Pseudomonas stutzeri
-
 L-tyrosine + NADH + CO2
-
 ?
1.3.1.43 L-arogenate + NAD+ Pseudomonas stutzeri JM300
-
 L-tyrosine + NADH + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.43 Pseudomonas stutzeri
-
 JM300
-
1.3.1.43 Pseudomonas stutzeri JM300
-
 JM300
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.43 recombinant His-tagged protein from Escherichia coli Pseudomonas stutzeri

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.1.43 L-arogenate + NAD+ = L-tyrosine + NADH + CO2 + H+ TyrA dehydrogenase superfamily Pseudomonas stutzeri
a
1.3.1.43 L-arogenate + NAD+ = L-tyrosine + NADH + CO2 + H+ substrate binding and inhibitory mechanism Pseudomonas stutzeri
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.43 L-arogenate + NAD(P)+
-
 Pseudomonas stutzeri L-tyrosine + NAD(P)H + CO2
-
 ?
1.3.1.43 L-arogenate + NAD(P)+
-
 Pseudomonas stutzeri JM300 L-tyrosine + NAD(P)H + CO2
-
 ?
1.3.1.43 L-arogenate + NAD+
-
 Pseudomonas stutzeri L-tyrosine + NADH + CO2
-
 ?
1.3.1.43 L-arogenate + NAD+
-
 Pseudomonas stutzeri JM300 L-tyrosine + NADH + CO2
-
 ?
1.3.1.43 additional information activity with prephenate and L-arogenate is unseparable during purification, thus cyclohexadienyl dehydrogenase activity Pseudomonas stutzeri ?
-
 ?
1.3.1.43 additional information activity with prephenate and L-arogenate is unseparable during purification, thus cyclohexadienyl dehydrogenase activity Pseudomonas stutzeri JM300 ?
-
 ?
1.3.1.43 prephenate + NAD(P)+
-
 Pseudomonas stutzeri ?
-
 ?
1.3.1.43 prephenate + NAD(P)+
-
 Pseudomonas stutzeri JM300 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.43 dimer 2 * 33000, recombinant His-tagged protein, forms a tetramer at high protein concentration, SDS-PAGE Pseudomonas stutzeri

Synonyms

EC Number Synonyms Comment Organism
1.3.1.43 cyclohexadienyl dehydrogenase
-
 Pseudomonas stutzeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.43 25
-
 assay at Pseudomonas stutzeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.43 7.1
-
-
 Pseudomonas stutzeri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.43 6 8
-
 Pseudomonas stutzeri

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.43 NAD+ only weak activity with NADP+, 0.25% of activity with NAD+ Pseudomonas stutzeri

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.1.43 0.23
-
 4-hydroxylphenylpyruvate
-
 Pseudomonas stutzeri
1.3.1.43 0.26
-
 L-tyrosine competitive inhibition Pseudomonas stutzeri