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Literature summary extracted from

  • Vick, B.A.; Zimmerman, D.C.
    Characterization of 12-oxo-phytodienoic acid reductase in corn (1986), Plant Physiol., 80, 202-205.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.42 0.013
-
 NADPH
-
 Zea mays
1.3.1.42 0.19
-
 12-oxophytodienoic acid
-
 Zea mays
1.3.1.42 4.2
-
 NADH
-
 Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.42 54000
-
 gel filtration Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.42 12-oxo-cis-phythodienoic acid + NADPH Zea mays third enzyme of the biosynthesis pathway of jasmonic acid from linolenic acid, enzyme catalyzes the reduction of a double bond in the cyclopentenone ring of 12-oxo-PDA cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.42 Zea mays
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.42 kernel
-
 Zea mays
-
1.3.1.42 seedling
-
 Zea mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.42 (15Z)-12-oxophyto-10,15-dienoate + NADH + H+
-
 Zea mays 8-[(1R,2R)-3-oxo-2-{(Z)-pent-2-enyl}cyclopentyl]octanoate + NAD+
-
 r
1.3.1.42 12-oxo-cis-phythodienoic acid + NADPH third enzyme of the biosynthesis pathway of jasmonic acid from linolenic acid, enzyme catalyzes the reduction of a double bond in the cyclopentenone ring of 12-oxo-PDA Zea mays cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+
-
 ?
1.3.1.42 12-oxo-trans-phythodienoic acid + NADPH
-
 Zea mays t-3-oxo-2-(2'-pentenyl)cyclopentaneoctanoic acid + NADP+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.42 6.8 8
-
 Zea mays

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.42 5 9
-
 Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.42 NADPH preferred reductant Zea mays