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Literature summary extracted from

  • Omori, T.; Ishigooka, H.; Minoda, Y.
    Purification and some properties of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) reducing enzyme from Pseudomonas cruciviae S93B1 involved in the degradation of biphenyl (1986), Agric. Biol. Chem., 50, 1513-1518.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.40 monoiodoacetate
-
 Pseudomonas cruciviae
1.3.1.40 p-chloromercuribenzoate
-
 Pseudomonas cruciviae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.40 170000
-
 2-hydroxy-6-oxo-phenylhexa-2,4-dienoate reducing enzyme III, gel filtration Pseudomonas cruciviae

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.40 Pseudomonas cruciviae
-
 S93B1, 2-hydroxy-6-oxo-phenylhexa-2,4-dienoate reducing enzymes I, II, III
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.40 2-hydroxy-6-oxo-phenylhexa-2,4-dienoate reducing enzyme III Pseudomonas cruciviae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.40 2.5
-
 2-hydroxy-6-oxo-phenylhexa-2,4-dienoate reducing enzyme III Pseudomonas cruciviae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.40 2-hydroxy-6-oxo-phenylhexa-2,4-dienoate + NADPH
-
 Pseudomonas cruciviae 2,6-dioxo-6-phenylhexanoate + NADP+
-
 ?
1.3.1.40 2-methoxy-6-oxo-6-phenylhexa-2,3-dienoic acid methyl ester + NADPH enzymes I and II Pseudomonas cruciviae 2-methoxy-6-oxo-6-phenylhexa-2-enoic acid methyl ester + NADP+
-
 ?
1.3.1.40 2-methoxy-6-oxo-6-phenylhexa-2,4-dienoic acid methyl ester + NADPH enzyme III Pseudomonas cruciviae 2-methoxy-6-oxo-6-phenylhexa-2-enoic acid methyl ester + NADP+
-
 ?
1.3.1.40 ring fission product of 3-methoxycatechol + NADPH enzymes I and II Pseudomonas cruciviae ?
-
 ?
1.3.1.40 ring fission products from 3-isopropylcatechol + NADPH enzymes I, II and III Pseudomonas cruciviae ?
-
 ?
1.3.1.40 ring fission products from 3-methylcatechol + NADPH enzymes I, II and III Pseudomonas cruciviae ?
-
 ?
1.3.1.40 ring fission products from biphenyl-2,3-diol + NADPH enzymes I, II and III Pseudomonas cruciviae ?
-
 ?
1.3.1.40 ring fission products of catechol + NADPH enzymes I and II Pseudomonas cruciviae ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.40 25
-
 assay at Pseudomonas cruciviae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.40 6
-
 2-hydroxy-6-oxo-phenylhexa-2,4-dienoate reducing enzyme III Pseudomonas cruciviae

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.40 NADPH
-
 Pseudomonas cruciviae