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Literature summary extracted from

  • Das, A.K.; Uhler, M.D.; Hajra, A.K.
    Molecular cloning and expression of mammalian peroxisomal trans-2-enoyl-coenzyme A reductase cDNAs (2000), J. Biol. Chem., 275, 24333-24340.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.38
-
 Homo sapiens
1.3.1.38
-
 Mus musculus
1.3.1.38 expression as His-tagged protein in Escherichia coli, sequence analysis Cavia porcellus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.38 N-ethylmaleimide native and recombinant enzyme Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.38 0.011
-
 trans-2-decenoyl-CoA
-
 Cavia porcellus
1.3.1.38 0.018
-
 trans-2-decenoyl-CoA recombinant enzyme Cavia porcellus
1.3.1.38 0.038
-
 NADPH
-
 Cavia porcellus
1.3.1.38 0.053
-
 NADPH recombinant enzyme Cavia porcellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.38 peroxisome
-
 Cavia porcellus 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.38 32300
-
 ? * 32300, sequence determination Homo sapiens
1.3.1.38 32600
-
 ? * 32600, sequence determination Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.38 additional information Cavia porcellus key enzyme in fatty acid chain elongation pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.38 Cavia porcellus Q9JIF5 guinea pig
-
1.3.1.38 Homo sapiens Q9BY49
-
-
1.3.1.38 Mus musculus Q99MZ7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.38 associated with peroxisomal acyldihydroxyacetone phosphate reductase Cavia porcellus
1.3.1.38 partial Cavia porcellus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.38 kidney
-
 Cavia porcellus
-
1.3.1.38 liver
-
 Homo sapiens
-
1.3.1.38 liver
-
 Cavia porcellus
-
1.3.1.38 liver
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.38 0.0752
-
 partially purified Cavia porcellus
1.3.1.38 0.346
-
 purified recombinant enzyme Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.38 additional information key enzyme in fatty acid chain elongation pathway Cavia porcellus ?
-
 ?
1.3.1.38 trans-2-decenoyl-CoA + NADPH highest activity with, native and recombinant enzyme Cavia porcellus decanoyl-CoA + NADP+
-
 ?
1.3.1.38 trans-2-hexenoyl-CoA + NADPH 50% activity compared to that with trans-2-decenoyl-CoA, native and recombinant enzyme Cavia porcellus hexanoyl-CoA + NADP+
-
 ?
1.3.1.38 trans-2-octenoyl-CoA + NADPH 50% activity compared to that with trans-2-decenoyl-CoA, native and recombinant enzyme Cavia porcellus octanoyl-CoA + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.38 ? ? * 32500-40000, sequence determination and SDS-PAGE Cavia porcellus
1.3.1.38 ? ? * 32600, sequence determination Mus musculus
1.3.1.38 ? ? * 32300, sequence determination Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.38 37
-
 assay at Cavia porcellus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.38 NADPH
-
 Homo sapiens
1.3.1.38 NADPH
-
 Mus musculus
1.3.1.38 NADPH specific for Cavia porcellus