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Literature summary extracted from

  • Seibert, V.; Stadler-Fritzsche, K.; Schloemann, M.
    Purification and characterization of maleylacetate reductase from Alcaligenes eutrophus JMP134(pJP4) (1993), J. Bacteriol., 175, 6745-6754.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.1.32 histidine histidine-HCl buffer results in considerably higher activity than corresponding bisTris-HCl or Tris-HCl buffers Cupriavidus necator

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.32
-
 Pseudomonas sp.

General Stability

EC Number General Stability Organism
1.3.1.32 addition of DTT significantly stabilized enzyme Cupriavidus necator

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.32 3-oxoadipate competitive inhibition Cupriavidus necator
1.3.1.32 Mn2+
-
 Cupriavidus necator
1.3.1.32 MnSO4
-
 Cupriavidus necator
1.3.1.32 p-chloromercuribenzoate
-
 Cupriavidus necator
1.3.1.32 p-chloromercuribenzoate
-
 Cutaneotrichosporon cutaneum
1.3.1.32 p-chloromercuribenzoate
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.32 0.024
-
 NADH 2-chloromaleylacetate as substrate Cupriavidus necator
1.3.1.32 0.031
-
 maleylacetate
-
 Cupriavidus necator
1.3.1.32 0.031
-
 2-chloromaleylacetate
-
 Cupriavidus necator
1.3.1.32 0.041
-
 NADH maleylacetate as substrate Cupriavidus necator
1.3.1.32 0.082
-
 NADPH 2-chloromaleylacetate as substrate Cupriavidus necator
1.3.1.32 0.089
-
 NADPH maleylacetate as substrate Cupriavidus necator
1.3.1.32 0.09
-
 maleylacetate NADPH as cosubstrate Cupriavidus necator
1.3.1.32 0.132
-
 2-chloromaleylacetate NADPH as cosubstrate Cupriavidus necator

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.32 18000
-
 strain PKO1 Pseudomonas sp.
1.3.1.32 35000
-
 2 * 35000, SDS-PAGE Cupriavidus necator
1.3.1.32 40000
-
 2 * 40000, SDS-PAGE Cutaneotrichosporon cutaneum
1.3.1.32 56000
-
 gel filtration, Superdex 200 Cupriavidus necator
1.3.1.32 72000
-
 analytical gel filtration Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.32 2-maleylacetate + NADPH Pseudomonas sp.
-
 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH Cupriavidus necator
-
 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH Cutaneotrichosporon cutaneum
-
 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH Sporotrichum pulverulentum
-
 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH Pseudomonas sp. B13
-
 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH Cupriavidus necator JMP 134-1
-
 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH Pseudomonas sp. PKO1
-
 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH Cupriavidus necator JMP 134(pJP4)
-
 3-oxoadipate + NADP+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.32 Cupriavidus necator
-
 JMP 134
-
1.3.1.32 Cupriavidus necator
-
 JMP 134(pJP4)
-
1.3.1.32 Cupriavidus necator JMP 134(pJP4)
-
 JMP 134(pJP4)
-
1.3.1.32 Cupriavidus necator JMP 134-1
-
 JMP 134
-
1.3.1.32 Cutaneotrichosporon cutaneum
-
-
-
1.3.1.32 Pseudomonas sp.
-
-
-
1.3.1.32 Pseudomonas sp. B13
-
-
-
1.3.1.32 Pseudomonas sp. PKO1
-
-
-
1.3.1.32 Sporotrichum pulverulentum
-
 lignin-degrading white-rot fungus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.32
-
 Pseudomonas sp.
1.3.1.32
-
 Cupriavidus necator
1.3.1.32
-
 Cutaneotrichosporon cutaneum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.32 600
-
-
 Cupriavidus necator

Storage Stability

EC Number Storage Stability Organism
1.3.1.32 -23°C, purified enzyme in 50 mM Tris-HCl, pH 7.5, 0.5 mM DTT, 0.1 mM EDTA after freezing, storage overnight and rethawing 35% loss of activity Cupriavidus necator
1.3.1.32 4°C, purified enzyme can be stored in 50 mM Tris-HCl, pH 7.5, 0.5 mM DTT, 0.1 mM EDTA with slight loss of activity, 36% in 3 weeks Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.32 2-chloromaleylacetate + NADPH reductive dehalogenation Cupriavidus necator 2-maleylacetate + Cl- + NADP+
-
 ?
1.3.1.32 2-chloromaleylacetate + NADPH reductive dehalogenation Cupriavidus necator JMP 134-1 2-maleylacetate + Cl- + NADP+
-
 ?
1.3.1.32 2-chloromaleylacetate + NADPH reductive dehalogenation Cupriavidus necator JMP 134(pJP4) 2-maleylacetate + Cl- + NADP+
-
 ?
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Pseudomonas sp. 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Cupriavidus necator 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Cutaneotrichosporon cutaneum 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Sporotrichum pulverulentum 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Pseudomonas sp. B13 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Cupriavidus necator JMP 134-1 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Pseudomonas sp. PKO1 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Cupriavidus necator JMP 134(pJP4) 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NADPH
-
 Pseudomonas sp. 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH
-
 Cupriavidus necator 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH
-
 Cutaneotrichosporon cutaneum 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH
-
 Sporotrichum pulverulentum 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH
-
 Pseudomonas sp. B13 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH
-
 Cupriavidus necator JMP 134-1 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH
-
 Pseudomonas sp. PKO1 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH
-
 Cupriavidus necator JMP 134(pJP4) 3-oxoadipate + NADP+
-
 ir
1.3.1.32 cis,cis-3-hydroxymuconate + NADPH
-
 Cupriavidus necator ? + NADP+
-
 ?
1.3.1.32 cis,cis-3-hydroxymuconate + NADPH
-
 Cupriavidus necator JMP 134-1 ? + NADP+
-
 ?
1.3.1.32 cis,cis-3-hydroxymuconate + NADPH
-
 Cupriavidus necator JMP 134(pJP4) ? + NADP+
-
 ?
1.3.1.32 trans,cis-3-hydroxymuconate + NADPH
-
 Cupriavidus necator ? + NADP+
-
 ?
1.3.1.32 trans,cis-3-hydroxymuconate + NADPH
-
 Cupriavidus necator JMP 134-1 ? + NADP+
-
 ?
1.3.1.32 trans,cis-3-hydroxymuconate + NADPH
-
 Cupriavidus necator JMP 134(pJP4) ? + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.32 dimer
-
 Pseudomonas sp.
1.3.1.32 dimer 2 * 35000, SDS-PAGE Cupriavidus necator
1.3.1.32 dimer 2 * 40000, SDS-PAGE Cutaneotrichosporon cutaneum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.32 6.5 7.8
-
 Cutaneotrichosporon cutaneum
1.3.1.32 7.5
-
-
 Cupriavidus necator

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.32 6 8.5
-
 Cupriavidus necator

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.3.1.32 6.5 8.5
-
 Cupriavidus necator

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.32 NADH
-
 Pseudomonas sp.
1.3.1.32 NADH
-
 Cutaneotrichosporon cutaneum
1.3.1.32 NADH NADH is preferred to NADPH Cupriavidus necator
1.3.1.32 NADPH
-
 Pseudomonas sp.
1.3.1.32 NADPH
-
 Cupriavidus necator
1.3.1.32 NADPH
-
 Cutaneotrichosporon cutaneum
1.3.1.32 NADPH
-
 Sporotrichum pulverulentum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.1.32 1.8
-
 3-oxoadipate
-
 Cupriavidus necator