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Literature summary extracted from

  • Gaal, A.B.; Neujahr, H.Y.
    Maleylacetate reductase from Trichosporon cutaneum (1980), Biochem. J., 185, 783-786.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.32 CuSO4 total inhibition, reversed by thiol Cutaneotrichosporon cutaneum
1.3.1.32 maleylacetate above 300 mM Cutaneotrichosporon cutaneum
1.3.1.32 p-hydroxymercuribenzoate total inhibition, reversed by thiol Cutaneotrichosporon cutaneum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.32 40000
-
 2 * 40000, SDS-PAGE Cutaneotrichosporon cutaneum
1.3.1.32 81000
-
 gel filtration Cutaneotrichosporon cutaneum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.32 2-maleylacetate + NADPH Pseudomonas putida
-
 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH Cutaneotrichosporon cutaneum
-
 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH Sporotrichum pulverulentum
-
 3-oxoadipate + NADP+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.32 Cutaneotrichosporon cutaneum
-
 phenol-utilizing yeast
-
1.3.1.32 Pseudomonas putida
-
-
-
1.3.1.32 Sporotrichum pulverulentum
-
 lignin-degrading white-rot fungus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.32
-
 Cutaneotrichosporon cutaneum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.32 34.2
-
-
 Cutaneotrichosporon cutaneum

Storage Stability

EC Number Storage Stability Organism
1.3.1.32 -25°C, 50 mM Tris/H2SO4 buffer, pH 7.6, can be stored for several months without loss of activity Cutaneotrichosporon cutaneum
1.3.1.32 5°C, 50 mM Tris/H2SO4 buffer, pH 7.6, can be stored for several days without loss of activity Cutaneotrichosporon cutaneum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Pseudomonas putida 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Cutaneotrichosporon cutaneum 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NAD(P)H
-
 Sporotrichum pulverulentum 3-oxoadipate + NAD(P)+
-
 ir
1.3.1.32 2-maleylacetate + NADPH
-
 Pseudomonas putida 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH
-
 Cutaneotrichosporon cutaneum 3-oxoadipate + NADP+
-
 ir
1.3.1.32 2-maleylacetate + NADPH
-
 Sporotrichum pulverulentum 3-oxoadipate + NADP+
-
 ir

Subunits

EC Number Subunits Comment Organism
1.3.1.32 dimer 2 * 40000, SDS-PAGE Cutaneotrichosporon cutaneum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.32 6.5 7.8
-
 Cutaneotrichosporon cutaneum

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.32 NADH
-
 Pseudomonas putida
1.3.1.32 NADH
-
 Cutaneotrichosporon cutaneum
1.3.1.32 NADPH
-
 Cutaneotrichosporon cutaneum
1.3.1.32 NADPH
-
 Sporotrichum pulverulentum