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Literature summary extracted from

  • Patel, T.R.; Gibson, D.T.
    Bacterial cis-dihydrodiol dehydrogenases: comparison of physicochemical and immunological protperties (1976), J. Bacteriol., 128, 842-850.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.29 0.011
-
 cis-1,2-dihydronaphthalene-1,2-diol
-
 Nocardia sp.
1.3.1.29 0.02
-
 cis-1,2-dihydronaphthalene-1,2-diol strain NP Pseudomonas putida
1.3.1.29 0.024
-
 cis-1,2-dihydronaphthalene-1,2-diol strain NCIB Pseudomonas putida
1.3.1.29 0.024
-
 cis-Toluene dihydrodiol strain biotype A grown on toluene Pseudomonas putida
1.3.1.29 0.05
-
 cis-benzene dihydrodiol strain biotype A grown on benzene Pseudomonas putida
1.3.1.29 0.071
-
 NAD+ strain biotype A grown on toluene Pseudomonas putida
1.3.1.29 0.079
-
 NAD+ strain biotype A grown on benzene Pseudomonas putida
1.3.1.29 0.65
-
 NAD+
-
 Nocardia sp.
1.3.1.29 1.02
-
 NAD+ strain NP Pseudomonas putida
1.3.1.29 1.04
-
 NAD+ strain NCIB Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.29 92000
-
 gel filtration Nocardia sp.
1.3.1.29 112000
-
 strain NCIB 9816, gel filtration Pseudomonas putida
1.3.1.29 155000 160000 biotype A, depending on growth medium, gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.29 Nocardia sp.
-
-
-
1.3.1.29 Pseudomonas putida
-
-
-
1.3.1.29 Pseudomonas putida
-
 strains NP, PL6 and C84-15
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.29 0.19
-
 activity in crude extracts Nocardia sp.
1.3.1.29 0.25
-
 activity in crude extracts of strain NP Pseudomonas putida
1.3.1.29 0.42
-
 activity in crude extracts of strain NCIB Pseudomonas putida
1.3.1.29 0.62
-
 activity in crude extracts of strain biotype A grown on benzene Pseudomonas putida
1.3.1.29 0.94
-
 activity in crude extracts of strain biotype A grown on toluene Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.29 cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+
-
 Pseudomonas putida 1,2-dihydroxynaphthalene + NADH
-
 ?
1.3.1.29 cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+
-
 Nocardia sp. 1,2-dihydroxynaphthalene + NADH
-
 ?
1.3.1.29 cis-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 Pseudomonas putida naphthalene-1,2-diol + NADH + H+
-
 r
1.3.1.29 cis-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 Nocardia sp. naphthalene-1,2-diol + NADH + H+
-
 r
1.3.1.29 cis-anthracene dihydrodiol + NAD+
-
 Pseudomonas putida 1,2-dihydroxyanthracene + NADH
-
 ?
1.3.1.29 cis-anthracene dihydrodiol + NAD+
-
 Nocardia sp. 1,2-dihydroxyanthracene + NADH
-
 ?
1.3.1.29 cis-benzene dihydrodiol + NAD+
-
 Pseudomonas putida 1,2-dihydroxybenzene + NADH
-
 ?
1.3.1.29 cis-benzene dihydrodiol + NAD+
-
 Nocardia sp. 1,2-dihydroxybenzene + NADH
-
 ?
1.3.1.29 cis-biphenyl dihydrodiol + NAD+
-
 Pseudomonas putida 3-phenyl-1,2-dihydroxybenzene + NADH
-
 ?
1.3.1.29 cis-biphenyl dihydrodiol + NAD+
-
 Nocardia sp. 3-phenyl-1,2-dihydroxybenzene + NADH
-
 ?
1.3.1.29 cis-ethylbenzene dihydrodiol + NAD+
-
 Pseudomonas putida 3-ethyl-1,2-dihydroxybenzene + NADH
-
 ?
1.3.1.29 cis-ethylbenzene dihydrodiol + NAD+
-
 Nocardia sp. 3-ethyl-1,2-dihydroxybenzene + NADH
-
 ?
1.3.1.29 cis-phenanthrene dihydrodiol + NAD+
-
 Pseudomonas putida 3,4-dihydroxyphenanthrene + NADH
-
 ?
1.3.1.29 cis-phenanthrene dihydrodiol + NAD+
-
 Nocardia sp. 3,4-dihydroxyphenanthrene + NADH
-
 ?
1.3.1.29 cis-toluene dihydrodiol + NAD+
-
 Pseudomonas putida 3-methyl-1,2-dihydroxybenzene + NADH
-
 ?
1.3.1.29 cis-toluene dihydrodiol + NAD+
-
 Nocardia sp. 3-methyl-1,2-dihydroxybenzene + NADH
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.1.29 50
-
 in cell extract: Pseudomonas putida NP stable, other Pseudomonas strains and Nocardia sp. unstable Pseudomonas putida
1.3.1.29 50
-
 in cell extract: Pseudomonas putida NP stable, other Pseudomonas strains and Nocardia sp. unstable Nocardia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.29 8.4
-
-
 Nocardia sp.
1.3.1.29 8.8 9
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.29 NAD+
-
 Pseudomonas putida
1.3.1.29 NAD+
-
 Nocardia sp.