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Literature summary extracted from
- Chorismate mutase/prephenate dehydrogenase from Escherichia coli K12: purification, characterization, and identification of a reactive cysteine (1984), Biochemistry, 23, 6240-6249.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.3.1.12 | glycerol stabilizes | Escherichia coli |
| 1.3.1.12 | unstable in buffers of low ionic strength lacking glycerol | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.1.12 | 5,5'-dithiobis(2-nitrobenzoate) | - |
Escherichia coli |  |
| 1.3.1.12 | iodoacetamide | - |
Escherichia coli | |
| 1.3.1.12 | iodoacetate | - |
Escherichia coli | |
| 1.3.1.12 | L-tyrosine | - |
Escherichia coli | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.3.1.12 | 0.045 | 0.09 | prephenate | - |
Escherichia coli | |
| 1.3.1.12 | 0.13 | 0.15 | NAD+ | - |
Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.12 | 78000 | 100000 | chorismate mutase-prephenate dehydrogenase bifunctional enzyme, sedimentation equilibrium centrifugation | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.1.12 | prephenate + NAD+ | Escherichia coli | biosynthesis of L-tyrosine | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.3.1.12 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.3.1.12 | chorismate mutase-prephenate dehydrogenase bifunctional enzyme | Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.12 | 52 | 101 | - |
Escherichia coli |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.3.1.12 | -20°C, 50 mM Cl/N-ethylmorpholine pH 7.2, 1 mM EDTA, 1 mM sodium citrate, 0.02% w/v sodium azide, 50% v/v glycerol, 10 mM DTT, N2-atmosphere, at least 3 months | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.1.12 | prephenate + NAD+ | - |
Escherichia coli | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? | |
| 1.3.1.12 | prephenate + NAD+ | biosynthesis of L-tyrosine | Escherichia coli | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.3.1.12 | dimer | 2 * 39000-42000, SDS-PAGE | Escherichia coli |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.3.1.12 | chorismate mutase-prephenate dehydrogenase bifunctional enzyme | complex with EC 5.4.99.5 | Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.3.1.12 | 135 | - |
prephenate | - |
Escherichia coli | |
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