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Literature summary extracted from

  • Weeks, G.; Wakil, S.J.
    Enoyl acyl carrier protein reductase from Escherichia coli (1969), Methods Enzymol., 14, 66-73.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.10 iodoacetate
-
 Escherichia coli
1.3.1.10 p-hydroxymercuribenzoate
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.10 additional information Escherichia coli part of fatty acid synthesis reducing double bonds of a great varity of acyl thioesters ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.10 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.10 no separation from NADH specific enzyme Escherichia coli
1.3.1.10 sonification and chromatography using DEAE-Cellulose, Sephadex G-100, Hydroxylapatite Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.10 2.31
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.3.1.10 -20°C, 10 mM phosphate buffer, pH 6.5, 5 mM 2-mercaptoethanol, inactivation in 2-3 months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.10 2-hexenoyl-[acyl-carrier protein] + NADPH
-
 Escherichia coli hexanoyl-[acyl-carrier protein] + NADP+
-
 ?
1.3.1.10 crotonyl-[acyl-carrier protein] + NADPH
-
 Escherichia coli butyryl-[acyl-carrier protein] + NADP+
-
 ?
1.3.1.10 additional information active on substrates with acyl chain length C4-C16 Escherichia coli ?
-
 ?
1.3.1.10 additional information inactive with enoyl-CoA substrates Escherichia coli ?
-
 ?
1.3.1.10 additional information part of fatty acid synthesis reducing double bonds of a great varity of acyl thioesters Escherichia coli ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.1.10 55
-
 5 min stable Escherichia coli
1.3.1.10 60
-
 5 min, inactivation Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.10 6
-
 no measurements below pH 6 carried out Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.10 6
-
 inactive above pH 8, no measurement below pH 6 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.3.1.10 7.6
-
 unstable at Escherichia coli