BRENDA - Enzyme Database

Effects of groESL coexpression on the folding of nicotinoprotein formaldehyde dismutase from Pseudomonas putida F61

Yanase, H.; Moriya, K.; Mukai, N.; Kawata, Y.; Okamoto, K.; Kato, N.; Biosci. Biotechnol. Biochem. 66, 85-91 (2002)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.2.98.1
coexpression in Escherichia coli with GroESL
Pseudomonas putida
Crystallization (Commentary)
EC Number
Crystallization
Organism
1.2.98.1
recombinant enzyme
Pseudomonas putida
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.98.1
Zinc
-
Pseudomonas putida
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.98.1
44000
-
4 * 44000, SDS-PAGE
Pseudomonas putida
1.2.98.1
180000
-
-
Pseudomonas putida
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.98.1
Pseudomonas putida
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.98.1
-
Pseudomonas putida
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.98.1
177
-
recombinant enzyme coexpressed with GroESL
Pseudomonas putida
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.98.1
1-butanol + p-nitroso-N,N-dimethylaniline
-
390495
Pseudomonas putida
n-butanal + reduced p-nitroso-N,N-dimethylaniline
-
390495
Pseudomonas putida
-
1.2.98.1
1-pentanol + p-nitroso-N,N-dimethylaniline
-
390495
Pseudomonas putida
n-pentanal + reduced p-nitroso-N,N-dimethylaniline
-
390495
Pseudomonas putida
-
1.2.98.1
1-propanol + p-nitroso-N,N-dimethylaniline
-
390495
Pseudomonas putida
n-propanal + reduced p-nitroso-N,N-dimethylaniline
-
390495
Pseudomonas putida
-
1.2.98.1
cyclohexanol + p-nitroso-N,N-dimethylaniline
50% of activity with ethanol
390495
Pseudomonas putida
cyclohexanal + reduced p-nitroso-N,N-dimethylaniline
-
390495
Pseudomonas putida
?
1.2.98.1
ethanol + p-nitroso-N,N-dimethylaniline
-
390495
Pseudomonas putida
ethanal + reduced p-nitroso-N,N-dimethylaniline
-
390495
Pseudomonas putida
-
1.2.98.1
n-hexanol + p-nitroso-N,N-dimethylaniline
14% of activity with ethanol
390495
Pseudomonas putida
n-hexanal + reduced p-nitroso-N,N-dimethylaniline
-
390495
Pseudomonas putida
?
Subunits
EC Number
Subunits
Commentary
Organism
1.2.98.1
tetramer
4 * 44000, SDS-PAGE
Pseudomonas putida
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.98.1
coexpression in Escherichia coli with GroESL
Pseudomonas putida
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
1.2.98.1
recombinant enzyme
Pseudomonas putida
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.98.1
Zinc
-
Pseudomonas putida
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.98.1
44000
-
4 * 44000, SDS-PAGE
Pseudomonas putida
1.2.98.1
180000
-
-
Pseudomonas putida
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.98.1
-
Pseudomonas putida
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.98.1
177
-
recombinant enzyme coexpressed with GroESL
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.98.1
1-butanol + p-nitroso-N,N-dimethylaniline
-
390495
Pseudomonas putida
n-butanal + reduced p-nitroso-N,N-dimethylaniline
-
390495
Pseudomonas putida
-
1.2.98.1
1-pentanol + p-nitroso-N,N-dimethylaniline
-
390495
Pseudomonas putida
n-pentanal + reduced p-nitroso-N,N-dimethylaniline
-
390495
Pseudomonas putida
-
1.2.98.1
1-propanol + p-nitroso-N,N-dimethylaniline
-
390495
Pseudomonas putida
n-propanal + reduced p-nitroso-N,N-dimethylaniline
-
390495
Pseudomonas putida
-
1.2.98.1
cyclohexanol + p-nitroso-N,N-dimethylaniline
50% of activity with ethanol
390495
Pseudomonas putida
cyclohexanal + reduced p-nitroso-N,N-dimethylaniline
-
390495
Pseudomonas putida
?
1.2.98.1
ethanol + p-nitroso-N,N-dimethylaniline
-
390495
Pseudomonas putida
ethanal + reduced p-nitroso-N,N-dimethylaniline
-
390495
Pseudomonas putida
-
1.2.98.1
n-hexanol + p-nitroso-N,N-dimethylaniline
14% of activity with ethanol
390495
Pseudomonas putida
n-hexanal + reduced p-nitroso-N,N-dimethylaniline
-
390495
Pseudomonas putida
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.98.1
tetramer
4 * 44000, SDS-PAGE
Pseudomonas putida