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Literature summary extracted from

  • Kato, N.; Yamagami, T.; Shimao, M.; Sakazawa, C.
    Formaldehyde dismutase, a novel NAD-binding oxidoreductase from Pseudomonas putida F61 (1986), Eur. J. Biochem., 156, 59-64.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.98.1 Pseudomonas putida F61 Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.98.1 HgCl2
-
 Pseudomonas putida
1.2.98.1 additional information not inhibited by AMP, ADP, ATP and N-methylnicotinamide chloride Pseudomonas putida
1.2.98.1 pyrazole
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.98.1 0.0014
-
 1-butanol + p-nitroso-N,N-dimethylaniline Pseudomonas putida
1.2.98.1 0.0032
-
 1-propanol + p-nitroso-N,N-dimethylaniline Pseudomonas putida
1.2.98.1 0.02
-
 ethanol + p-nitroso-N,N-dimethylaniline Pseudomonas putida
1.2.98.1 0.04
-
 methanol + p-nitroso-N,N-dimethylaniline Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.98.1 Zinc each subunit of the tetramer contains 2 mol zinc per mol Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.98.1 44000
-
 4 * 44000, SDS-PAGE Pseudomonas putida
1.2.98.1 180000
-
 gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.2.98.1 Pseudomonas putida
-
 F61
-
1.2.98.1 Pseudomonas putida F61
-
 F61
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.98.1
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.98.1 1-butanol + p-nitroso-N,N-dimethylaniline 96.2% of activity with ethanol or methanol Pseudomonas putida n-butanal + reduced p-nitroso-N,N-dimethylaniline
-
 ?
1.2.98.1 1-butanol + p-nitroso-N,N-dimethylaniline 96.2% of activity with ethanol or methanol Pseudomonas putida F61 n-butanal + reduced p-nitroso-N,N-dimethylaniline
-
 ?
1.2.98.1 1-pentanol + p-nitroso-N,N-dimethylaniline 9.62% of activity with ethanol Pseudomonas putida n-pentanal + reduced p-nitroso-N,N-dimethylaniline
-
 ?
1.2.98.1 1-pentanol + p-nitroso-N,N-dimethylaniline 9.62% of activity with ethanol Pseudomonas putida F61 n-pentanal + reduced p-nitroso-N,N-dimethylaniline
-
 ?
1.2.98.1 1-propanol + p-nitroso-N,N-dimethylaniline
-
 Pseudomonas putida n-propanal + reduced p-nitroso-N,N-dimethylaniline
-
 ?
1.2.98.1 1-propanol + p-nitroso-N,N-dimethylaniline
-
 Pseudomonas putida F61 n-propanal + reduced p-nitroso-N,N-dimethylaniline
-
 ?
1.2.98.1 ethanol + p-nitroso-N,N-dimethylaniline
-
 Pseudomonas putida ethanal + reduced p-nitroso-N,N-dimethylaniline
-
 ?
1.2.98.1 ethanol + p-nitroso-N,N-dimethylaniline
-
 Pseudomonas putida F61 ethanal + reduced p-nitroso-N,N-dimethylaniline
-
 ?
1.2.98.1 formaldehyde + H2O dismutation Pseudomonas putida formate + methanol
-
 ?
1.2.98.1 formaldehyde + H2O dismutation Pseudomonas putida F61 formate + methanol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.98.1 tetramer 4 * 44000, SDS-PAGE Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.98.1 NADH each subunit contains 1 mol NADH per mol, binds firmly at each active site, enzyme-bound NAD(H) is reduced and oxidized only by the added second substrate (alcohol, aldehyde) not by exogenous electron acceptor Pseudomonas putida