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Literature summary extracted from

  • Lu, W.P.; Ragsdale, S.W.
    Reductive activation of the coenzyme A/acetyl-CoA isotopic exchange reaction catalyzed by carbon monoxide dehydrogenase from Clostridium thermoaceticum and its inhibition by nitrous oxide and carbon monoxide (1991), J. Biol. Chem., 266, 3554-3564.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.169 CO two effects: stimulation and inhibition on CoA/acetylCoA exchange Moorella thermoacetica

General Stability

EC Number General Stability Organism
2.3.1.169 reactions in presence of DTT, since enzyme requires strictly anaerobic conditions for stability Moorella thermoacetica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.7.4 CN- 1.2 mM, 98% inhibition of acetyl-CoA/CoA exchange reaction Moorella thermoacetica
1.2.7.4 CO inhibition of the exchange reaction between acetyl-CoA and CoA Moorella thermoacetica
1.2.7.4 N2O inhibition of the exchange reaction between acetyl-CoA and CoA Moorella thermoacetica
2.3.1.169 CN- inhibitor on the CoA/acetyl-CoA exchange, 98% inhibition at 1.2 mM Moorella thermoacetica
2.3.1.169 CO non-competitive inhibitor on the CoA/acetyl-CoA exchange, the Ni-Fe-C-center appears to be the inhibitor site for CO Moorella thermoacetica
2.3.1.169 CO2 inhibitor on the CoA/acetyl-CoA exchange Moorella thermoacetica
2.3.1.169 dephospho-CoA inhibitor on the CoA/acetyl-CoA exchange, 75% inhibition at 0.44 mM Moorella thermoacetica
2.3.1.169 desulfo-CoA inhibitor on the CoA/acetyl-CoA exchange, 30% mM at 2.1 mM Moorella thermoacetica
2.3.1.169 N2O inhibitor on the CoA/acetyl-CoA exchange Moorella thermoacetica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.169 Fe corrinoid/iron-sulfur protein required Moorella thermoacetica
2.3.1.169 Fe the enzyme-bound complex can be described as an [NiFe3-4S4]-acetyl complex Moorella thermoacetica
2.3.1.169 Ni the enzyme-bound complex can be described as an [NiFe3-4S4]-acetyl complex Moorella thermoacetica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.169 150000
-
-
Moorella thermoacetica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.4 CO + H2O + electron acceptor Moorella thermoacetica the enzyme is involved in the Wood pathway of acetyl-CoA synthesis ?
-
?
2.3.1.169 additional information Moorella thermoacetica key enzyme in the autotrophic acetyl-CoA pathway, i.e. Wood pathway, enzyme catalyses the final steps in this pathway ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.4 Moorella thermoacetica
-
-
-
2.3.1.169 Moorella thermoacetica
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.169 acetyl-CoA + a [Co(I) corrinoid Fe-S protein] = CO + CoA + a [methyl-Co(III) corrinoid Fe-S protein] pathway Moorella thermoacetica
2.3.1.169 acetyl-CoA + a [Co(I) corrinoid Fe-S protein] = CO + CoA + a [methyl-Co(III) corrinoid Fe-S protein] Enzyme accepts the methyl group from the methylated corrinoid/iron-sulfur protein, binds a carbonyl group from CO, CO2, or the carboxyl of pyruvate, and binds coenzyme A. Then the enzyme catalyses the synthesis of acetyl-CoA from these enzyme bound groups. Additionally the enzyme catalyses two exchange reactions between the methylated corrinoid/iron-sulfur protein and methylated enzyme and between methylated enzyme and the methyl moiety of acetyl-CoA. Moorella thermoacetica
2.3.1.169 acetyl-CoA + a [Co(I) corrinoid Fe-S protein] = CO + CoA + a [methyl-Co(III) corrinoid Fe-S protein] the enzyme-bound complex is an [NiFe3-4S4]-acetyl complex Moorella thermoacetica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.4 7
-
acetyl-CoA/CoA exchange reaction at pH 6.0 and 45°C, 100% CO in the gas phase Moorella thermoacetica
1.2.7.4 28
-
acetyl-CoA/CoA exchange reaction at pH 6.0 and 45°C, CO in the gas phase replaced by N2 Moorella thermoacetica
2.3.1.169 28
-
40°C, CoA/acetyl-CoA exchange Moorella thermoacetica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.4 CO + H2O + electron acceptor the enzyme is involved in the Wood pathway of acetyl-CoA synthesis Moorella thermoacetica ?
-
?
1.2.7.4 CO + H2O + FMN
-
Moorella thermoacetica CO2 + FMNH2
-
?
1.2.7.4 CO + H2O + methyl viologen catalyzes the exchange reaction between coenzyme A and acetyl-CoA Moorella thermoacetica CO2 + reduced methyl viologen
-
?
2.3.1.169 CH3-(corrinoid/iron-sulfur protein) + CO + HS-CoA under anaerobic conditions Moorella thermoacetica CH3-CO-S-CoA + corrinoid/iron-sulfur protein
-
?
2.3.1.169 CO + methyl-X + HS-CoA
-
Moorella thermoacetica CH3-CO-S-CoA + HX
-
?
2.3.1.169 additional information enzyme catalyses the CoA/acetyl-CoA exchange Moorella thermoacetica ?
-
?
2.3.1.169 additional information key enzyme in the autotrophic acetyl-CoA pathway, i.e. Wood pathway, enzyme catalyses the final steps in this pathway Moorella thermoacetica ?
-
?

Subunits

EC Number Subunits Comment Organism
2.3.1.169 dimer alpha,beta Moorella thermoacetica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.169 additional information
-
additional information
-
Moorella thermoacetica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.169 6.7 7 optimum for CoA/acetyl-CoA exchange Moorella thermoacetica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.7.4 0.4
-
CO inhibition of acetyl-CoA/CoA exchange reaction Moorella thermoacetica
2.3.1.169 0.4
-
CO pH 7.0, 25°C, under anaerobic conditions, noncompetitive with respect to acetyl-CoA Moorella thermoacetica