EC Number | General Stability | Organism |
---|---|---|
1.2.7.4 | 72 h exposure to air at 4°C, about 10% loss of activity | Methanothrix soehngenii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.7.4 | CN- | 0.2 mM, 17% inhibition in the presence of air, 32% CO and 69% N2, inhibition can be removed by O2 or CO | Methanothrix soehngenii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.7.4 | 0.065 | - |
methyl viologen | - |
Methanothrix soehngenii | |
1.2.7.4 | 0.7 | - |
CO | - |
Methanothrix soehngenii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.7.4 | 19400 | - |
2 * 19400 + 2 * 79400, SDS-PAGE | Methanothrix soehngenii |
1.2.7.4 | 79400 | - |
2 * 19400 + 2 * 79400, SDS-PAGE | Methanothrix soehngenii |
1.2.7.4 | 190000 | - |
gel filtration | Methanothrix soehngenii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.7.4 | Methanothrix soehngenii | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.2.7.4 | oxygen stable, 10% loss in activity after 72 h exposure to air at 4°C | Methanothrix soehngenii |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.7.4 | - |
Methanothrix soehngenii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.7.4 | 117 | - |
- |
Methanothrix soehngenii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.4 | CO + H2O + coenzyme F420 | 1.5% of the activity with methyl viologen | Methanothrix soehngenii | CO2 + reduced coenzyme F420 | - |
? | |
1.2.7.4 | CO + H2O + FAD | 0.3% of the activity with methyl viologen | Methanothrix soehngenii | CO2 + FADH2 | - |
? | |
1.2.7.4 | CO + H2O + FMN | 0.6% of the activity with methyl viologen | Methanothrix soehngenii | CO2 + FMNH2 | - |
? | |
1.2.7.4 | CO + H2O + methyl viologen | - |
Methanothrix soehngenii | CO2 + reduced methyl viologen | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.7.4 | tetramer | 2 * 19400 + 2 * 79400, SDS-PAGE | Methanothrix soehngenii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.7.4 | 60 | - |
10 min, about 10% loss of activity | Methanothrix soehngenii |
1.2.7.4 | 72 | - |
10 min, complete inactivation | Methanothrix soehngenii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.7.4 | 9 | - |
- |
Methanothrix soehngenii |