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Literature summary extracted from

  • Ragsdale, S.W.; Wood, H.G.
    Acetate biosynthesis by acetogenic bacteria. Evidence that carbon monoxide dehydrogenase is the condensing enzyme that catalyzes the final steps of the synthesis (1985), J. Biol. Chem., 260, 3970-3977.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.169 additional information neither ATP nor phosphate stimulate the exchange Moorella thermoacetica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.7.4 5,5'-dithiobis (2-nitrobenzoate)
-
 Moorella thermoacetica
1.2.7.4 Mersalyl acid
-
 Moorella thermoacetica
1.2.7.4 methyl iodide
-
 Moorella thermoacetica
1.2.7.4 additional information phenylglyoxal, methylglyoxal, butanedione, mersalyl acid, methyl iodide, 5,5'-dithiobis (2-nitrobenzoate) and sodium dithionite inhibits the exchange reaction between CO and acetyl-CoA Moorella thermoacetica
2.3.1.169 5,5'dithiobis-(2-nitrobenzoic acid) inhibits the acetyl-CoA/CO exchange reaction Moorella thermoacetica
2.3.1.169 Mersalyl acid inhibits the acetyl-CoA/CO exchange reaction Moorella thermoacetica
2.3.1.169 methyl iodide inhibits the acetyl-CoA/CO exchange reaction Moorella thermoacetica
2.3.1.169 additional information no inhibition of the exchange reaction by methyl- and phenylglyoxal, and butanedione Moorella thermoacetica
2.3.1.169 Sodium dithionite inhibits the acetyl-CoA/CO exchange reaction Moorella thermoacetica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.4 CO + H2O + electron acceptor Moorella thermoacetica key role of the enzyme in the synthesis of acetyl-CoA ?
-
 ?
1.2.7.4 CO + H2O + oxidized ferredoxin Moorella thermoacetica acetate biosynthesis pathway, catalyzes an exchange reaction between CO and the carbonyl group of acetyl-CoA CO2 + reduced ferredoxin
-
 ?
2.3.1.169 additional information Moorella thermoacetica the purified carbon monoxide dehydrogenase, EC 1.2.7.4, from Clostridium thermoaceticum is the only protein required to catalyze a reversible exchange reaction between carbon monoxide and the carbonyl group of acetyl-CoA. Carbon dioxide also exchanges with the C-1 of acetyl-coA, but at a much lower rate than does CO ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.4 Moorella thermoacetica
-
-
-
1.2.7.4 Moorella thermoacetica
-
 formerly Clostridium thermoaceticum
-
2.3.1.169 Moorella thermoacetica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.4
-
 Moorella thermoacetica
2.3.1.169 native enzyme by anion exchange and hydrophobic interaction chromatography, followed by hydroxylapatite chromatography, gel filtration, and CoA affinity chromatography Moorella thermoacetica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.169 additional information
-
 70 mol of CO exchanged per min/mol of enzyme Moorella thermoacetica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.4 CO + H2O + electron acceptor key role of the enzyme in the synthesis of acetyl-CoA Moorella thermoacetica ?
-
 ?
1.2.7.4 CO + H2O + methyl viologen catalyzes at a low rate an exchange between CO2 and the carbonyl group of acetyl-CoA Moorella thermoacetica CO2 + reduced methyl viologen
-
 ?
1.2.7.4 CO + H2O + methyl viologen catalyzes an exchange reaction between CO and the carbonyl group of acetyl-CoA Moorella thermoacetica CO2 + reduced methyl viologen
-
 ?
1.2.7.4 CO + H2O + oxidized ferredoxin
-
 Moorella thermoacetica CO2 + reduced ferredoxin
-
 ?
1.2.7.4 CO + H2O + oxidized ferredoxin acetate biosynthesis pathway, catalyzes an exchange reaction between CO and the carbonyl group of acetyl-CoA Moorella thermoacetica CO2 + reduced ferredoxin
-
 ?
2.3.1.169 additional information the purified carbon monoxide dehydrogenase, EC 1.2.7.4, from Clostridium thermoaceticum is the only protein required to catalyze a reversible exchange reaction between carbon monoxide and the carbonyl group of acetyl-CoA. Carbon dioxide also exchanges with the C-1 of acetyl-coA, but at a much lower rate than does CO Moorella thermoacetica ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.169 50
-
-
 Moorella thermoacetica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.7.4 additional information
-
 additional information pH 5.3, 50°C, activity of CO oxidation to CO2, ferredoxin II as electron carrier, turnover number is approximately 780 mol/mol of CO dehydrogenase Moorella thermoacetica
1.2.7.4 1.17
-
 CO turnover mumber for CO/CO2 exchange reaction Moorella thermoacetica
1.2.7.4 13
-
 CO CO oxidation, 50°C, pH 5.3, electron acceptor ferredoxin II Moorella thermoacetica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.4 5.3
-
-
 Moorella thermoacetica
2.3.1.169 5.3
-
-
 Moorella thermoacetica