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Literature summary extracted from

  • Drake, H.L.; Hu, S.I.; Wood, H.G.
    Purification of carbon monoxide dehydrogenase, a nickel enzyme from Clostridium thermoaceticum (1980), J. Biol. Chem., 255, 7174-7180.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.7.4 CN- KCN, reversible Moorella thermoacetica
1.2.7.4 KCN CO reverses cyanide inhibition, but promotes reaction with methyl iodide Moorella thermoacetica
1.2.7.4 additional information not affected by propyl iodide, methyl iodide, carbon tetrachloride,or metal chelators; not inhibited by propyl iodide, methyl iodide, carbon tetrachloride, EDTA or nitrilotriacetate Moorella thermoacetica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.4 Ni2+
-
 Moorella thermoacetica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.4 410000
-
 gel filtration Moorella thermoacetica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.4 CO + H2O + electron acceptor Moorella thermoacetica ferredoxin and a membrane-bound b-type cytochrome are considered to be the native electron carriers ?
-
 ?
1.2.7.4 CO + H2O + oxidized cytochrome b Moorella thermoacetica membrane-bound b-type, native electron carrier CO2 + reduced cytochrome b
-
 ?
1.2.7.4 CO + H2O + oxidized ferredoxin Moorella thermoacetica
-
 CO2 + reduced ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.4 Moorella thermoacetica
-
-
-
1.2.7.4 Moorella thermoacetica
-
 formerly Clostridium thermoaceticum
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.2.7.4 enzymatic activity is destroyed by air, a low amount of activity still is present after 2 h exposure to air Moorella thermoacetica

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.4
-
 Moorella thermoacetica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.4 additional information
-
 method for in situ assay Moorella thermoacetica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.4 CO + H2O + cytochrome b
-
 Moorella thermoacetica CO2 + reduced cytochrome b
-
 ?
1.2.7.4 CO + H2O + cytochrome c3 cytochrome 3 from Desulfovibrio vulgaris Moorella thermoacetica CO2 + reduced cytochrome c3
-
 ?
1.2.7.4 CO + H2O + electron acceptor ferredoxin and a membrane-bound b-type cytochrome are considered to be the native electron carriers Moorella thermoacetica ?
-
 ?
1.2.7.4 CO + H2O + ferredoxin active with ferredoxin obtained from Clostridium thermoaceticum, no activity with ferredoxin from spinach Moorella thermoacetica CO2 + reduced ferredoxin
-
 ?
1.2.7.4 CO + H2O + FMN
-
 Moorella thermoacetica CO2 + FMNH2
-
 ?
1.2.7.4 CO + H2O + methyl viologen no activity with FAD, NAD+ and NADP+ Moorella thermoacetica CO2 + reduced methyl viologen
-
 ?
1.2.7.4 CO + H2O + oxidized cytochrome b membrane-bound b-type, native electron carrier Moorella thermoacetica CO2 + reduced cytochrome b
-
 ?
1.2.7.4 CO + H2O + oxidized cytochrome c3 Desulfovibrio vulgaris cytochrome c3 Moorella thermoacetica CO2 + reduced cytochrome c3
-
 ?
1.2.7.4 CO + H2O + oxidized ferredoxin
-
 Moorella thermoacetica CO2 + reduced ferredoxin
-
 ?
1.2.7.4 CO + H2O + oxidized ferredoxin ferredoxin from Clostridium pasteurianum is also a substrate Moorella thermoacetica CO2 + reduced ferredoxin
-
 ?
1.2.7.4 CO + H2O + oxidized flavodoxin
-
 Moorella thermoacetica CO2 + reduced flavodoxin
-
 ?
1.2.7.4 CO + H2O + oxidized methyl viologen
-
 Moorella thermoacetica CO2 + reduced methyl viologen
-
 ?
1.2.7.4 additional information spinach ferredoxin, FAD, NAD+ and NADP+ are not reduced Moorella thermoacetica ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.4 70
-
-
 Moorella thermoacetica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.4 10.6
-
-
 Moorella thermoacetica
1.2.7.4 10.6
-
 metyl viologen as substrate Moorella thermoacetica