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Literature summary extracted from

  • Nagasawa, T.; Kawabata, Y.; Tani, Y.; Ogata, K.
    Purification and characterization of betaine aldehyde dehydrogenase from Pseudomonas aeruginosa A-16 (1976), Agric. Biol. Chem., 40, 1743-1749.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.2.1.8 dialysis for 5 days against 0.1 M buffer, pH 7.4, at 4°C, stable Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.8 1,10-phenanthroline no inhibition at 1 mM Pseudomonas aeruginosa
1.2.1.8 2,2'-dipyridyl no inhibition at 3 mM Pseudomonas aeruginosa
1.2.1.8 acetaldehyde 10 mM, 69% inhibition Pseudomonas aeruginosa
1.2.1.8 Acetylcholine 10 mM, 18% inhibition Pseudomonas aeruginosa
1.2.1.8 AgNO3 3 mM, 90-100% inhibition Pseudomonas aeruginosa
1.2.1.8 benzyltrimethylamine iodide 10 mM, 45% inhibition Pseudomonas aeruginosa
1.2.1.8 betaine 10 mM, 14% inhibition Pseudomonas aeruginosa
1.2.1.8 choline 10 mM, 23% inhibition Pseudomonas aeruginosa
1.2.1.8 Glutaraldehyde 10 mM, 83% inhibition Pseudomonas aeruginosa
1.2.1.8 Hg2+ 3 mM HgCl2, 90-100% inhibition Pseudomonas aeruginosa
1.2.1.8 iodoacetate 1 mM Pseudomonas aeruginosa
1.2.1.8 Isobutanal 10 mM, 93% inhibition Pseudomonas aeruginosa
1.2.1.8 n-butylaldehyde 10 mM, 96% inhibition Pseudomonas aeruginosa
1.2.1.8 NH4+
-
 Pseudomonas aeruginosa
1.2.1.8 PCMB 0.01 mM Pseudomonas aeruginosa
1.2.1.8 tetraethylamine iodide 10 mM, 19% inhibition Pseudomonas aeruginosa
1.2.1.8 tetramethylamine iodide 10 mM, 19% inhibition Pseudomonas aeruginosa
1.2.1.8 tetrapropylamine iodide 10 mM, 43% inhibition Pseudomonas aeruginosa
1.2.1.8 ZnCl2 3 mM, 90-100% inhibition Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.8 0.089
-
 NADP+
-
 Pseudomonas aeruginosa
1.2.1.8 0.22
-
 NAD+
-
 Pseudomonas aeruginosa
1.2.1.8 0.38
-
 Betaine aldehyde
-
 Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.8 145000
-
 gel filtration Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD+ + H2O Pseudomonas aeruginosa inducible enzyme accumulates in the presence of choline, acetylcholine or betaine in the medium betaine + NADH
-
 ?
1.2.1.8 betaine aldehyde + NAD+ + H2O Pseudomonas aeruginosa A-16 inducible enzyme accumulates in the presence of choline, acetylcholine or betaine in the medium betaine + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.8 Pseudomonas aeruginosa
-
 A-16
-
1.2.1.8 Pseudomonas aeruginosa A-16
-
 A-16
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.8
-
 Pseudomonas aeruginosa

Storage Stability

EC Number Storage Stability Organism
1.2.1.8 -20°C, 9.1 M buffer, pH 7.4, 3 weeks, stable Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD+ + H2O reverse reaction not detected Pseudomonas aeruginosa betaine + NADH
-
 ir
1.2.1.8 betaine aldehyde + NAD+ + H2O inducible enzyme accumulates in the presence of choline, acetylcholine or betaine in the medium Pseudomonas aeruginosa betaine + NADH
-
 ?
1.2.1.8 betaine aldehyde + NAD+ + H2O reverse reaction not detected Pseudomonas aeruginosa A-16 betaine + NADH
-
 ir
1.2.1.8 betaine aldehyde + NAD+ + H2O inducible enzyme accumulates in the presence of choline, acetylcholine or betaine in the medium Pseudomonas aeruginosa A-16 betaine + NADH
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.8 40
-
 pH 7.4, 0.01% 2-mercaptoethanol, 7 min, 50% loss of activity Pseudomonas aeruginosa
1.2.1.8 50
-
 pH 7.4, 0.01% 2-mercaptoethanol, 7 min, complete loss of activity Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.8 9
-
-
 Pseudomonas aeruginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.8 7 9.5 pH 7.0: about 25% of maximal activity, pH 9.5: about 50% of maximal activity Pseudomonas aeruginosa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.8 4.5
-
 15 h, 80% loss of activity Pseudomonas aeruginosa
1.2.1.8 5 7.5 15 h, stable Pseudomonas aeruginosa
1.2.1.8 8
-
 15 h, 60% loss of activity Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.8 NAD+
-
 Pseudomonas aeruginosa
1.2.1.8 NADP+
-
 Pseudomonas aeruginosa