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Literature summary extracted from

  • Inoue, J.; Shaw, J.P.; Rekik, M.; Harayama, S.
    Overlapping substrate specificities of benzaldehyde dehydrogenase (the xylC gene product) and 2-hydroxymuconic semialdehyde dehydrogenase (the xylG gene product) encoded by TOL plasmid pWW0 of Pseudomonas putida (1995), J. Bacteriol., 177, 1196-1201.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.2.1.28 dithiothreitol stabilizes Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.28 0.0015
-
 4-methylbenzaldehyde
-
 Pseudomonas putida
1.2.1.28 0.0019
-
 3-methylbenzaldehyde
-
 Pseudomonas putida
1.2.1.28 0.0021
-
 3-Chlorobenzaldehyde
-
 Pseudomonas putida
1.2.1.28 0.0025
-
 benzaldehyde
-
 Pseudomonas putida
1.2.1.28 0.0026
-
 3-Methoxybenzaldehyde
-
 Pseudomonas putida
1.2.1.28 0.0029
-
 3-Fluorobenzaldehyde
-
 Pseudomonas putida
1.2.1.28 0.022
-
 3-Nitrobenzaldehyde
-
 Pseudomonas putida
1.2.1.28 0.32
-
 NAD+
-
 Pseudomonas putida
1.2.1.32 0.0093
-
 2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate synonym 5-methyl-2-hydroxymuconic-6-semialdehyde Pseudomonas putida
1.2.1.32 0.017
-
 2-hydroxymuconate-6-semialdehyde
-
 Pseudomonas putida
1.2.1.32 0.17
-
 3-Fluorobenzaldehyde
-
 Pseudomonas putida
1.2.1.32 0.33
-
 NAD+
-
 Pseudomonas putida
1.2.1.32 0.46
-
 benzaldehyde
-
 Pseudomonas putida
1.2.1.32 0.53
-
 4-methylbenzaldehyde
-
 Pseudomonas putida
1.2.1.32 0.63
-
 3-methylbenzaldehyde
-
 Pseudomonas putida
1.2.1.32 0.81
-
 3-Nitrobenzaldehyde
-
 Pseudomonas putida
1.2.1.32 0.94
-
 3-Methoxybenzaldehyde
-
 Pseudomonas putida
1.2.1.32 1.3
-
 3-Chlorobenzaldehyde
-
 Pseudomonas putida
1.2.1.85 0.0093
-
 2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate in 100 mM Tris-HCl, pH 8.5, at 25°C Pseudomonas putida
1.2.1.85 0.017
-
 2-hydroxymuconate-6-semialdehyde in 100 mM Tris-HCl, pH 8.5, at 25°C Pseudomonas putida
1.2.1.85 0.17
-
 3-Fluorobenzaldehyde in 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
1.2.1.85 0.33
-
 NAD+ in the presence of benzaldehyde, 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
1.2.1.85 0.46
-
 benzaldehyde in 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
1.2.1.85 0.53
-
 4-methylbenzaldehyde in 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
1.2.1.85 0.63
-
 3-methylbenzaldehyde in 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
1.2.1.85 0.81
-
 3-Nitrobenzaldehyde in 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
1.2.1.85 0.94
-
 3-Methoxybenzaldehyde in 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
1.2.1.85 1.3
-
 3-Chlorobenzaldehyde in 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.28 51941
-
 4 * 51941, deduced from nucleotide sequence Pseudomonas putida
1.2.1.28 52500
-
 4 * 52500, SDS-PAGE Pseudomonas putida
1.2.1.28 122000 126000 gel filtration Pseudomonas putida
1.2.1.28 208000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.28 benzaldehyde + NAD+ + H2O Pseudomonas putida
-
 benzoate + NADH + H+
-
 ?
1.2.1.28 benzaldehyde + NAD+ + H2O Pseudomonas putida KT 2240
-
 benzoate + NADH + H+
-
 ?
1.2.1.32 2-hydroxymuconic acid semialdehyde + NAD+ + H2O Pseudomonas putida
-
 2-hydroxymuconate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.28 Pseudomonas putida
-
-
-
1.2.1.32 Pseudomonas putida
-
-
-
1.2.1.85 Pseudomonas putida
-
-
-
1.2.1.85 Pseudomonas putida KT 2240
-
-
-
1.13.11.2 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.28
-
 Pseudomonas putida
1.2.1.32 erroneous identification as benzaldehyde dehydrogenase corrected Pseudomonas putida
1.2.1.85 ammonium sulfate fractionation, TSKgel DEAE-5PW column chromatography, Affigel-Blue affinity chromatography, and TSKgel Phenyl-5PW column chromatography Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.28 16
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.28 3-chlorobenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 3-chlorobenzoate + NADH
-
 ?
1.2.1.28 3-chlorobenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida KT 2240 3-chlorobenzoate + NADH
-
 ?
1.2.1.28 3-fluorobenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 3-fluorobenzoate + NADH + H+
-
 ?
1.2.1.28 3-methoxybenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 3-methoxybenzoate + NADH
-
 ?
1.2.1.28 3-methylbenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 3-methylbenzoate + NADH
-
 ?
1.2.1.28 3-nitrobenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 3-nitrobenzoate + NADH
-
 ?
1.2.1.28 3-nitrobenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida KT 2240 3-nitrobenzoate + NADH
-
 ?
1.2.1.28 4-carboxybenzaldehyde + NAD+ + H2O 1650% of activity with benzaldehyde Pseudomonas putida 4-carboxybenzoate + NADH
-
 ?
1.2.1.28 4-carboxybenzaldehyde + NAD+ + H2O 1650% of activity with benzaldehyde Pseudomonas putida KT 2240 4-carboxybenzoate + NADH
-
 ?
1.2.1.28 4-methoxybenzaldehyde + NAD+ + H2O 90% of activity with benzaldehyde Pseudomonas putida 4-methoxybenzoate + NADH + H+
-
 ?
1.2.1.28 4-methylbenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 4-methylbenzoate + NADH
-
 ?
1.2.1.28 benzaldehyde + NAD+ + H2O
-
 Pseudomonas putida benzoate + NADH + H+
-
 ?
1.2.1.28 benzaldehyde + NAD+ + H2O
-
 Pseudomonas putida benzoate + NADH + H+
-
 ir
1.2.1.28 benzaldehyde + NAD+ + H2O
-
 Pseudomonas putida KT 2240 benzoate + NADH + H+
-
 ?
1.2.1.28 benzaldehyde + NAD+ + H2O
-
 Pseudomonas putida KT 2240 benzoate + NADH + H+
-
 ir
1.2.1.28 pyridine 4-carboxyaldehyde + NAD+ + H2O 1380% of activity with benzaldehyde Pseudomonas putida pyridine 4-formiate + NADH
-
 ?
1.2.1.32 2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate + NAD+ + H2O synonym 5-methyl-2-hydroxymuconic-6-semialdehyde Pseudomonas putida 2-hydroxy-5-methylmuconate + NADH
-
 ?
1.2.1.32 2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
-
 Pseudomonas putida 2-hydroxymuconate + NADH
-
 ?
1.2.1.32 2-hydroxymuconic acid semialdehyde + NAD+ + H2O
-
 Pseudomonas putida 2-hydroxymuconate + NADH + H+
-
 ?
1.2.1.32 3-chlorobenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 3-chlorobenzoate + NADH
-
 ?
1.2.1.32 3-fluorobenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 3-fluorobenzoate + NADH + H+
-
 ?
1.2.1.32 3-methoxybenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 3-methoxybenzoate + NADH
-
 ?
1.2.1.32 3-methylbenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 3-methylbenzoate + NADH
-
 ?
1.2.1.32 3-nitrobenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 3-nitrobenzoate + NADH
-
 ?
1.2.1.32 4-methylbenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 4-methylbenzoate + NADH
-
 ?
1.2.1.32 benzaldehyde + NAD+ + H2O low activity Pseudomonas putida benzoate + NADH + H+
-
 ?
1.2.1.85 2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate + NAD+ + H2O
-
 Pseudomonas putida (2E,4Z)-2-hydroxy-5-methylhexa-2,4-diendioate + NADH + H+
-
 ?
1.2.1.85 2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
-
 Pseudomonas putida (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + H+
-
 ?
1.2.1.85 3-chlorobenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 3-chlorobenzoate + NADH + H+
-
 ?
1.2.1.85 3-fluorobenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 3-fluorobenzoate + NADH + H+
-
 ?
1.2.1.85 3-methoxybenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 3-methoxybenzoate + NADH + H+
-
 ?
1.2.1.85 3-methylbenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 3-methylbenzoate + NADH + H+
-
 ?
1.2.1.85 3-nitrobenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 3-nitrobenzoate + NADH + H+
-
 ?
1.2.1.85 4-methylbenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 4-methylbenzoate + NADH + H+
-
 ?
1.2.1.85 benzaldehyde + NAD+ + H2O weak substrate Pseudomonas putida benzoate + NADH + H+
-
 ?
1.13.11.2 4-methylcatechol + O2
-
 Pseudomonas putida 2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.28 tetramer 4 * 52500, SDS-PAGE Pseudomonas putida
1.2.1.28 tetramer 4 * 51941, deduced from nucleotide sequence Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.2.1.85 2-hydroxymuconic semialdehyde dehydrogenase
-
 Pseudomonas putida
1.2.1.85 XylG
-
 Pseudomonas putida
1.13.11.2 XylE
-
 Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.28 9
-
-
 Pseudomonas putida
1.2.1.85 8.3
-
 pH optimum for the oxidation of 2-hydroxymuconic semialdehyde Pseudomonas putida
1.2.1.85 9.6
-
 pH optimum for the oxidation of benzaldehyde Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.28 6 10
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.28 NAD+
-
 Pseudomonas putida
1.2.1.32 NAD+
-
 Pseudomonas putida

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.85 8
-
 3-Methoxybenzaldehyde apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
1.2.1.85 10
-
 4-methylbenzaldehyde apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
1.2.1.85 20
-
 3-Nitrobenzaldehyde apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
1.2.1.85 22
-
 3-Chlorobenzaldehyde apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
1.2.1.85 32
-
 benzaldehyde apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
1.2.1.85 36
-
 3-methylbenzaldehyde apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
1.2.1.85 56
-
 NAD+ apparent value, in the presence of benzaldehyde, 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
1.2.1.85 330
-
 3-Fluorobenzaldehyde apparent value, in 100 mM glycine-NaOH (pH 9.2), at 25°C Pseudomonas putida
1.2.1.85 1600
-
 2-hydroxymuconate-6-semialdehyde apparent value, in 100 mM Tris-HCl, pH 8.5, at 25°C Pseudomonas putida
1.2.1.85 2100
-
 2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate apparent value, in 100 mM Tris-HCl, pH 8.5, at 25°C Pseudomonas putida