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Literature summary extracted from

  • Sokatch, J.R.; Sanders, L.E.; Marshall, V.P.
    Oxidation of methylmalonate semialdehyde to propionyl coenzyme A in Pseudomonas aeruginosa grown on valine (1968), J. Biol. Chem., 243, 2500-2506.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.27 2-methyl-3-oxopropanoate + CoA + NAD+ Pseudomonas aeruginosa
-
 propanoyl-CoA + CO2 + NADH
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.27 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.27 partial Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.27 2-methyl-3-oxopropanoate + CoA + NAD+
-
 Pseudomonas aeruginosa propanoyl-CoA + CO2 + NADH
-
 ir
1.2.1.27 2-methyl-3-oxopropanoate + CoA + NAD+ other acyl acceptor: 2-mercaptoethanol Pseudomonas aeruginosa propanoyl-CoA + CO2 + NADH
-
 ir
1.2.1.27 propanal + 2-mercaptoethanol + NAD+
-
 Pseudomonas aeruginosa ? + NADH
-
 ir
1.2.1.27 propanal + CoA + NAD+
-
 Pseudomonas aeruginosa propanoyl-CoA + NADH
-
 ir

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.27 8.5
-
 2-methyl-3-oxopropanoate + CoA + NAD+ Pseudomonas aeruginosa
1.2.1.27 8.5
-
 2-methyl-3-oxopropanoate + 2-mercaptoethanol + NAD+ Pseudomonas aeruginosa
1.2.1.27 8.5
-
 propanal + 2-mercaptoethanol + NAD+ Pseudomonas aeruginosa
1.2.1.27 9
-
 propanal + CoA + NAD+ Pseudomonas aeruginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.27 7 10 methylmalonate semialdehyde oxidation, more than 90% of maximal activity Pseudomonas aeruginosa
1.2.1.27 8 10 propanal oxidation, less than 50% of maximal activity below, less than 60% of maximal activity above Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.27 CoA
-
 Pseudomonas aeruginosa
1.2.1.27 NAD+
-
 Pseudomonas aeruginosa