Literature summary extracted from

Quayle, J.R.; Taylor, G.A.
Carbon assimilation by Pseudomonas oxalaticus (OX1) (1961), Biochem. J., 78, 611-615.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.2.1.17	Tris buffer	-	Cupriavidus oxalaticus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.2.1.17	glyoxylate + CoA + NADP+	Pseudomonas sp.	-	oxalyl-CoA + NADPH	-	r
1.2.1.17	glyoxylate + CoA + NADP+	Cupriavidus oxalaticus	-	oxalyl-CoA + NADPH	-	r

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.2.1.17	Cupriavidus oxalaticus	-	-	-
1.2.1.17	Pseudomonas sp.	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.2.1.17	using protamine sulfate treatment, ammonium sulfate precipitation and dialysis, alumina C-gamma adsorption, concentration of the enzyme by ammonium sulfate precipitation followed by dialysis and ion exchange chromatography on DEAE-cellulose	Cupriavidus oxalaticus

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.2.1.17	4.2	-	-	Cupriavidus oxalaticus
1.2.1.17	7.2	-	-	Cupriavidus oxalaticus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.2.1.17	glyoxylate + CoA + NADP+	-	Pseudomonas sp.	oxalyl-CoA + NADPH	-	r
1.2.1.17	glyoxylate + CoA + NADP+	-	Cupriavidus oxalaticus	oxalyl-CoA + NADPH	-	r

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.2.1.17	8.6	-	glyoxylate + CoA + NADP+	Cupriavidus oxalaticus

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.2.1.17	CoA	-	Pseudomonas sp.
1.2.1.17	CoA	specific for	Cupriavidus oxalaticus
1.2.1.17	NADP+	-	Pseudomonas sp.
1.2.1.17	NADP+	specific for	Cupriavidus oxalaticus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)