Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sanchez, L.B.
    Aldehyde dehydrogenase (CoA-acetylating) and the mechanism of ethanol formation in the amitochondriate protist, Giardia lamblia (1998), Arch. Biochem. Biophys., 354, 57-64.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.10 Ca2+ 5 mM causes 51% inhibition Giardia intestinalis
1.2.1.10 Disulfiram 0.01 mM causes 70% inhibition Giardia intestinalis
1.2.1.10 iodoacetamide 1 mM causes 88-96% inhibition Giardia intestinalis
1.2.1.10 Mg2+ 5 mM causes 51% inhibition Giardia intestinalis
1.2.1.10 Mn2+ 5 mM causes 84% inhibition Giardia intestinalis
1.2.1.10 p-chloromercuribenzoate 0.01 mM causes 88-96% inhibition Giardia intestinalis
1.2.1.10 Valproate 50 mM causes 20-46% inhibition Giardia intestinalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.10 0.018
-
 acetyl-CoA
-
 Giardia intestinalis
1.2.1.10 0.022
-
 NADH
-
 Giardia intestinalis
1.2.1.10 0.076
-
 CoA
-
 Giardia intestinalis
1.2.1.10 0.362
-
 acetaldehyde
-
 Giardia intestinalis
1.2.1.10 0.603
-
 propanal
-
 Giardia intestinalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.10 cytosol
-
 Giardia intestinalis 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.10 95000
-
 x * 95000, SDS-PAGE, amino acid sequence Giardia intestinalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.10 acetaldehyde + CoA + NAD+ Giardia intestinalis
-
 acetyl-CoA + NADH
-
 r
1.2.1.10 acetaldehyde + CoA + NAD+ Giardia intestinalis WB / CC 30957
-
 acetyl-CoA + NADH
-
 r
1.2.1.10 propanal + CoA + NAD+ Giardia intestinalis is used 63% as efficiently as acetaldehyde propionyl-CoA + NADH
-
 r
1.2.1.10 propanal + CoA + NAD+ Giardia intestinalis WB / CC 30957 is used 63% as efficiently as acetaldehyde propionyl-CoA + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.10 Giardia intestinalis
-
 srain WB
-
1.2.1.10 Giardia intestinalis WB / CC 30957
-
 srain WB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.10
-
 Giardia intestinalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.10 8.75
-
 bifunctional fusion protein of aldehyde and alcohol dehydrogenase Giardia intestinalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.10 acetaldehyde + CoA + NAD+
-
 Giardia intestinalis acetyl-CoA + NADH
-
 r
1.2.1.10 acetaldehyde + CoA + NAD+ enzyme carries aldehyde and alcohol dehydrogenase activity Giardia intestinalis acetyl-CoA + NADH
-
 r
1.2.1.10 acetaldehyde + CoA + NAD+
-
 Giardia intestinalis WB / CC 30957 acetyl-CoA + NADH
-
 r
1.2.1.10 acetaldehyde + CoA + NAD+ enzyme carries aldehyde and alcohol dehydrogenase activity Giardia intestinalis WB / CC 30957 acetyl-CoA + NADH
-
 r
1.2.1.10 formaldehyde + CoA + NAD+
-
 Giardia intestinalis formyl-CoA + NADH
-
 ir
1.2.1.10 isobutyraldehyde + CoA + NAD+
-
 Giardia intestinalis isobutyryl-CoA + NADH
-
 r
1.2.1.10 isobutyraldehyde + CoA + NAD+
-
 Giardia intestinalis WB / CC 30957 isobutyryl-CoA + NADH
-
 r
1.2.1.10 n-butyraldehyde + CoA + NAD+
-
 Giardia intestinalis n-butyryl-CoA + NADH
-
 ?
1.2.1.10 n-butyraldehyde + CoA + NAD+
-
 Giardia intestinalis WB / CC 30957 n-butyryl-CoA + NADH
-
 ?
1.2.1.10 propanal + CoA + NAD+ is used 63% as efficiently as acetaldehyde Giardia intestinalis propionyl-CoA + NADH
-
 r
1.2.1.10 propanal + CoA + NAD+ is used 63% as efficiently as acetaldehyde Giardia intestinalis WB / CC 30957 propionyl-CoA + NADH
-
 r

Subunits

EC Number Subunits Comment Organism
1.2.1.10 ? x * 95000, SDS-PAGE, amino acid sequence Giardia intestinalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.10 8.5
-
 NAD+ reduction Giardia intestinalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.10 CoA
-
 Giardia intestinalis
1.2.1.10 NAD+ NAD+-specific Giardia intestinalis
1.2.1.10 NAD+ no reaction with NADP+ Giardia intestinalis