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Literature summary extracted from

  • Yan, R.T.; Chen, J.S.
    Coenzyme A-acylating aldehyde dehydrogenase from Clostridium beijerinckii NRRL B592 (1990), Appl. Environ. Microbiol., 56, 2591-2599.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.2.1.10 sensitive to O2, can be protected against O2 inactivation by dithiothreitol Clostridium beijerinckii
1.2.1.10 with Tris acetate buffer at pH 7, less stable at higher buffer concentration between 10 and 150 mM Clostridium beijerinckii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.10 NAD+ double competitive, NAD+/CoA ratio is kept at 1:0.24 Clostridium beijerinckii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.10 0.0076
-
 NADH forward reaction, with butyryl-CoA Clostridium beijerinckii
1.2.1.10 0.0082
-
 NADH forward reaction, with acetyl-CoA Clostridium beijerinckii
1.2.1.10 0.0196
-
 acetyl-CoA forward reaction, with NADPH Clostridium beijerinckii
1.2.1.10 0.064
-
 CoA reverse reaction, with 0.32 mM NAD+ and 11 mM butyraldehyde Clostridium beijerinckii
1.2.1.10 0.0673
-
 NADPH forward reaction, with butyryl-CoA Clostridium beijerinckii
1.2.1.10 0.0715
-
 n-butyryl-CoA forward reaction, with NADPH Clostridium beijerinckii
1.2.1.10 0.154
-
 acetyl-CoA forward reaction, with NADH Clostridium beijerinckii
1.2.1.10 0.166
-
 n-butyryl-CoA forward reaction, with NADH Clostridium beijerinckii
1.2.1.10 0.206
-
 NADPH forward reaction, with acetyl-CoA Clostridium beijerinckii
1.2.1.10 0.25
-
 NAD+ reverse reaction, with 0.12 mM CoA and 11 mM butyraldehyde Clostridium beijerinckii
1.2.1.10 3.7
-
 Butyraldehyde reverse reaction, with 0.32 mM NAD+ and 0.12 mM CoA Clostridium beijerinckii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.10 55000
-
 2 * 55000, SDS-PAGE Clostridium beijerinckii
1.2.1.10 100000
-
 gel filtration under anaerobic conditions Clostridium beijerinckii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.10 acetaldehyde + CoA + NAD+ Clostridium beijerinckii
-
 acetyl-CoA + NADH
-
 r
1.2.1.10 acetaldehyde + CoA + NAD+ Clostridium beijerinckii NRRL B592
-
 acetyl-CoA + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.10 Clostridium beijerinckii
-
-
-
1.2.1.10 Clostridium beijerinckii NRRL B592
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.10 under anaerobic conditions Clostridium beijerinckii

Reaction

EC Number Reaction Comment Organism Reaction ID
1.2.1.10 acetaldehyde + CoA + NAD+ = acetyl-CoA + NADH + H+ bi-uni-uni-uni-ping-pong mechanism Clostridium beijerinckii
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.10 2
-
-
 Clostridium beijerinckii
1.2.1.10 4.88
-
 undialyzed, anaerobically purified Clostridium beijerinckii
1.2.1.10 9.62
-
 dialyzed, incubated with dithiothreitol and CoA Clostridium beijerinckii

Storage Stability

EC Number Storage Stability Organism
1.2.1.10 5 mM dithiothreitol protects enzyme in crude extracts from O2-inactivation for at least 2 hours Clostridium beijerinckii
1.2.1.10 addition of 140 mM KCl to 10 mM Tris acetate stabilizes as no activity is lost after 3 days Clostridium beijerinckii
1.2.1.10 CoA and dithiothreitol restore a higher activity than one of these compounds alone Clostridium beijerinckii
1.2.1.10 glycerol at 20%, vol/vol, stabilizes in 50 mM Tris acetate buffer at pH 7, as no activity is lost after 3 days at 4°C under argon Clostridium beijerinckii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.10 acetaldehyde + CoA + NAD+
-
 Clostridium beijerinckii acetyl-CoA + NADH
-
 r
1.2.1.10 acetaldehyde + CoA + NAD+
-
 Clostridium beijerinckii NRRL B592 acetyl-CoA + NADH
-
 r
1.2.1.10 acetyl-CoA + NADPH + H+
-
 Clostridium beijerinckii acetaldehyde + CoA + NADP+
-
 r
1.2.1.10 acetyl-CoA + NADPH + H+
-
 Clostridium beijerinckii NRRL B592 acetaldehyde + CoA + NADP+
-
 r
1.2.1.10 butyryl-CoA + NADPH + H+
-
 Clostridium beijerinckii butyraldehyde + CoA + NADP+
-
 r
1.2.1.10 butyryl-CoA + NADPH + H+
-
 Clostridium beijerinckii NRRL B592 butyraldehyde + CoA + NADP+
-
 r
1.2.1.10 n-butyraldehyde + CoA + NAD+
-
 Clostridium beijerinckii n-butyryl-CoA + NADH
-
 r
1.2.1.10 n-butyraldehyde + CoA + NAD+
-
 Clostridium beijerinckii NRRL B592 n-butyryl-CoA + NADH
-
 r

Subunits

EC Number Subunits Comment Organism
1.2.1.10 dimer 2 * 55000, SDS-PAGE Clostridium beijerinckii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.10 7
-
-
 Clostridium beijerinckii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.10 6 7 NAD+ reduction Clostridium beijerinckii
1.2.1.10 6.5 9.5 activity of NADH oxidation increases between 6.5-9.5 Clostridium beijerinckii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.10 7
-
 more stable at pH 7 than at pH 6, 8 or 9 in either Tris acetate or potassium phosphate test buffer, at the same pH more stable in Tris acetate test buffer Clostridium beijerinckii

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.10 CoA
-
 Clostridium beijerinckii
1.2.1.10 NAD+
-
 Clostridium beijerinckii
1.2.1.10 NADH shows also activity with NADPH, NADH is more effective than NADPH Clostridium beijerinckii