Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Auchus, R.J.; Miller, W.L.
    Molecular modeling of human P450c17 (17alpha-hydroxylase/17,20-lyase): insights into reaction mechanisms and effects of mutations (1999), Mol. Endocrinol., 13, 1169-1182.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.14.19 cytochrome b5 acts as allosteric facilitator but not as electron donor, increases 17,20-lyase activity Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.19 expression in Saccharomyces cerevisiae strain W303B of the K89N-mutant Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.19 K89N 78% loss of 17,20-lyase activity and 20% loss of 17alpha-hydroxylase activity Homo sapiens
1.14.14.19 R347H loss of 17,20-lyase activity to a greater extent than 17alpha-hydroxylase activity Homo sapiens
1.14.14.19 R358Q loss of 17,20-lyase activity to a greater extent than 17alpha-hydroxylase activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.19 17-hydroxypregnenolone competitive inhibitor of 17alpha-hydrolase activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.19 0.0004
-
 17-hydroxypregnenolone wild type enzyme Homo sapiens
1.14.14.19 0.0008
-
 17-hydroxypregnenolone K89N mutant expressed in Saccharomyces cerevisiae Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.19 microsome
-
 Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.19 Fe3+ heme Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.19 additional information Homo sapiens it catalyzes steroid 17alpha-hydroxylase and 17,20-lyase activities in the biosynthesis of androgens, estrogens and cortisol ?
-
 ?
1.14.14.19 pregnenolone + AH2 + O2 Homo sapiens
-
 17alpha-hydroxypregnenolone + A + H2O product eliminates at C20,21 acetate to yield dehydroepiandrosterone ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.19 Homo sapiens P05093
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.19 additional information both the lyase and hydroxylase activities proceed from a common steroid-binding geometry by an iron oxene mechanism Homo sapiens ?
-
 ?
1.14.14.19 additional information it catalyzes steroid 17alpha-hydroxylase and 17,20-lyase activities in the biosynthesis of androgens, estrogens and cortisol Homo sapiens ?
-
 ?
1.14.14.19 pregnenolone + AH2 + O2
-
 Homo sapiens 17alpha-hydroxypregnenolone + A + H2O product eliminates at C20,21 acetate to yield dehydroepiandrosterone ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.19 P450c17
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.19 NADPH
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.14.19 0.0003
-
 17-hydroxypregnenolone R347H-mutant, inhibiton of 17alpha-hydrolase activity Homo sapiens
1.14.14.19 0.0005
-
 17-hydroxypregnenolone K89N-mutant, inhibiton of 17alpha-hydrolase activity Homo sapiens
1.14.14.19 0.0007
-
 17-hydroxypregnenolone wild type, inhibiton of 17alpha-hydrolase activity Homo sapiens
1.14.14.19 0.0008
-
 17-hydroxypregnenolone R358Q-mutant, inhibiton of 17alpha-hydrolase activity Homo sapiens