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Literature summary extracted from

  • Nozaki, M.
    Imidazolacetate monooxygenase (Pseudomonas) (1971), Methods Enzymol., 17B, 773-777.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.13.5
-
 Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.5 mercury compounds
-
 Pseudomonas sp.
1.14.13.5 p-chloromercuribenzoate
-
 Pseudomonas sp.
1.14.13.5 silver compounds
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.5 0.002
-
 NADH
-
 Pseudomonas sp.
1.14.13.5 0.002 0.01 NADH
-
 Pseudomonas sp.
1.14.13.5 0.02
-
 O2
-
 Pseudomonas sp.
1.14.13.5 0.3
-
 imidazoleacetate
-
 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.5 87000 90000 sedimentation equilibrium, gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.5 4-imidazoleacetate + NADH + O2 Pseudomonas sp.
-
 5-hydroxy-4-imidazoleacetate + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.5 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.5 using protamine sulfate treatment, ammonium sulfate fractionation, chromatography on TEAE-cellulose column, calcium phosphate gel treatment and chromatography on DEAE-Sephadex column Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.5 25
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.14.13.5 -20°C, potassium phosphate buffer, pH 7.2, no loss of activity for at least 1 month Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.5 4-imidazoleacetate + NAD(P)H + O2 + H+
-
 Pseudomonas sp. 5-hydroxy-4-imidazoleacetate + NAD(P)+ + H2O
-
 ?
1.14.13.5 4-imidazoleacetate + NADH + O2
-
 Pseudomonas sp. 5-hydroxy-4-imidazoleacetate + NAD+ + H2O
-
 ?
1.14.13.5 imidazolelactate + NADH + O2 + H+ 4% relative activity to 4-imidazoleacetate Pseudomonas sp. 5-hydroxyimidazolelactate + NAD+ + H2O
-
 ?
1.14.13.5 imidazolepropionate + NAD(P)H + O2 + H+ 10% relative activity to 4-imidazoleacetate Pseudomonas sp. 5-hydroxyimidazolepropionate + NAD(P)+ + H2O
-
 ?
1.14.13.5 sodium diethyldithiocarbamate + NADH + O2
-
 Pseudomonas sp. ? + NAD+ + H2O reaction product not identified ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.13.5 30
-
 50% loss of activity, 0.1 M potassium phosphate, pH 7.2, 5 min Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.5 9
-
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.5 FAD 1 mol per mol enzyme Pseudomonas sp.
1.14.13.5 NADH
-
 Pseudomonas sp.
1.14.13.5 NADPH beta-NADPH is approximately 13% as active as beta-NADH Pseudomonas sp.
1.14.13.5 NADPH less efficient than NADH Pseudomonas sp.